E3 ubiquitin ligase RNF6 promotes antiviral immune responses through enhancing the expression of interferon stimulated genes in myeloid cells

Wei Sun; Xin Hua; Yanli Gu; Qiuli Xu; Suhua Zhu; Tangfeng Lv; Yong Song

doi:10.1016/j.clim.2022.109099

E3 ubiquitin ligase RNF6 promotes antiviral immune responses through enhancing the expression of interferon stimulated genes in myeloid cells

Clin Immunol. 2022 Sep:242:109099. doi: 10.1016/j.clim.2022.109099. Epub 2022 Aug 13.

Authors

Wei Sun¹, Xin Hua², Yanli Gu³, Qiuli Xu², Suhua Zhu⁴, Tangfeng Lv⁵, Yong Song⁶

Affiliations

¹ Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical School of Southeast University, Nanjing, China; Department of Rheumatology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China.
² Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical School of Southeast University, Nanjing, China.
³ Department of Respiratory and Critical Care Medicine, Jinling Hospital, Nanjing Medical University, Nanjing, China.
⁴ Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.
⁵ Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical School of Southeast University, Nanjing, China; Department of Respiratory and Critical Care Medicine, Jinling Hospital, Nanjing Medical University, Nanjing, China; Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing, China. Electronic address: bairoushui@163.com.
⁶ Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical School of Southeast University, Nanjing, China; Department of Respiratory and Critical Care Medicine, Jinling Hospital, Nanjing Medical University, Nanjing, China; Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing, China. Electronic address: yong.song@nju.edu.cn.

PMID: 35973638
DOI: 10.1016/j.clim.2022.109099

Abstract

Interferon signaling is closely associated with clearance of viral infections as well as the development of systemic lupus erythematosus (SLE). Therefore, from a clinical perspective, it is important to identify the key regulators involved in interferon signaling pathways. In this study, we identified that RNF6, as an interferon inducible E3 ubiquitin ligase, promoted the interferon-dependent antiviral response. Knock-down of RNF6 greatly attenuated expression of ISGs and the transcriptional activity of ISRE. Specifically, increased RNF6 expression in myeloid cells of patients with SLE correlated with high expression of ISGs. Our results uncover RNF6 as a positive mediator in the antiviral immune responses and suggest that RNF6 may contribute to predict interferon signaling in SLE.

Keywords: Innate immunity; Myeloid cell; RNF6; Systemic lupus erythematosus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antiviral Agents
DNA-Binding Proteins / genetics
Humans
Immunity
Interferons
Lupus Erythematosus, Systemic*
Myeloid Cells / metabolism
Ubiquitin-Protein Ligases* / genetics

Substances

Antiviral Agents
DNA-Binding Proteins
RNF6 protein, human
Interferons
Ubiquitin-Protein Ligases