Duganella dendranthematis sp. nov. and Massilia forsythiae sp. nov., isolated from flowers

Abstract

Two aerobic, Gram-stain-negative, motile, mesophilic, rod-shaped and catalase-positive bacterial strains designated AF9R3^T and GN2-R2^T were isolated from flowers collected in the Republic of Korea. Strain AF9R3^T grew at 4-33 °C, pH 4.0-9.0 and with 0-1 % NaCl (w/v), and strain GN2-R2^T grew at 10-33 °C, pH 4.0-9.0 and with 0-1 % NaCl (w/v). Phylogenetic analysis on the basis of 16S rRNA gene sequences indicated that strains AF9R3^T and GN2-R2^T belonged to the genera Duganella and Massilia, respectively, showing high sequence similarity to Duganella levis CY42W^T (99.4 %) and Massilia putida 6 NM-7^T (98.0 %), respectively. Both strains contained summed feature 3 (C_{16 : 1} ω7c and/or C_{16 : 1} ω6c) and C_{16 : 0} as the major fatty acids, and ubiquinone Q-8 as the predominant quinone. Strain AF9R3^T had diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and strain GN2-R2^T comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid as the major polar lipids. Orthologous average nucleotide identity and digital DNA-DNA hybridization values of strain AF9R3^T to its closest relative D. levis CY42W^T were 92.6 and 56.5 %, and those of strain GN2-R2^T to its closest relative M. putida 6 NM-7^T were 81.4 and 24.8 %. Based on genotypic and phenotypic data, strains AF9R3^T and GN2-R2^T are considered to represent novel species of the genus Duganella and Massilia, respectively, for which the names Duganella dendranthematis sp. nov. (type strain AF9R3^T=KACC 21258^T=NBRC 114510^T) and Massilia forsythiae sp. nov. (type strain GN2-R2^T=KACC 21261^T=NBRC 114511^T) have been proposed.

Keywords: MIDI; menaquinone; novel species; polar lipid; strain AF9R3T; strain GN2-R2T.