Biogenic synthesis of selenium and tellurium nanoparticles by marine bacteria and their biological activity

I A Beleneva; U V Kharchenko; A D Kukhlevsky; A V Boroda; N V Izotov; A S Gnedenkov; V S Egorkin

doi:10.1007/s11274-022-03374-6

Biogenic synthesis of selenium and tellurium nanoparticles by marine bacteria and their biological activity

World J Microbiol Biotechnol. 2022 Aug 16;38(11):188. doi: 10.1007/s11274-022-03374-6.

Authors

I A Beleneva¹, U V Kharchenko², A D Kukhlevsky³, A V Boroda³, N V Izotov², A S Gnedenkov², V S Egorkin²

Affiliations

¹ A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Palchevskogo Str. 17, Vladivostok, Russia, 690041. beleneva.vl@mail.ru.
² Institute of Chemistry, Far Eastern Branch, Russian Academy of Sciences, Pr. 100-letiya Vladivostoka, 159, Vladivostok, Russia, 690022.
³ A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Palchevskogo Str. 17, Vladivostok, Russia, 690041.

PMID: 35972591
DOI: 10.1007/s11274-022-03374-6

Abstract

Selenium (SeNPs) and tellurium nanoparticles (TeNPs) were synthesized by green technology using the three new bacterial marine isolates (strains PL 2476, AF 2469 and G 2451). Isolates were classified as Pseudoalteromonas shioyasakiensis according to 16S rRNA sequence analysis, morphological characteristics, and biochemical reactions. The bioreduction processes of isolates were studied in comparison with the previously described Alteromonas macleodii (strain 2328). All strains exhibited significant tolerance to selenite and tellurite up to 1000 µg/mL. A comparative analysis of the bioreduction processes of the isolates demonstrated that the strains have a high rate of reduction processes. Characterization of biogenic red SeNPs and black TeNPs using scanning electron microscopy (SEM), EDX analysis, Dynamic Light Scattering, and micro-Raman Spectroscopy revealed that all the isolates form stable spherical selenium and tellurium nanoparticles whose size as well as elemental composition depend on the producer strain. Nanoparticles of the smallest size (up to 100 nm) were observed only for strain PL 2476. Biogenic SeNPs and TeNPs were also characterized and tested for their antimicrobial, antifouling and cytotoxic activities. Significant antimicrobial activity was shown for nanoparticles at relatively high concentrations (500 and 1000 µg/mL), with the antimicrobial activity of TeNPs being more significant than SeNPs. In contrast, against cell cultures (breast cancer cells (SkBr3) and human dermal fibroblasts (HDF) SeNPs showed greater toxicity than tellurium nanoparticles. Studies have demonstrated the high antifouling effectiveness of selenium and tellurium nanoparticles when introduced into self-polishing coatings. According to the results obtained, the use of SeNPs and TeNPs as antifouling additives can reduce the concentration of leachable biocides used in coatings, reducing the pressure on the environment.

Keywords: Antibacterial; Antifouling activity; Bioactivity; Biogenic nanostructures; Biosynthesis; Cytotoxicity; SeNPs; TeNPs.

MeSH terms

Bacteria
Humans
Nanoparticles* / chemistry
RNA, Ribosomal, 16S / genetics
Selenium* / pharmacology
Tellurium / chemistry
Tellurium / pharmacology

Substances

RNA, Ribosomal, 16S
Selenium
Tellurium