Ca2Fe2O5 powder antifungal activity to the Candida utilis culture upon its growth

Abstract

This study reports the impact of Ca₂Fe₂O₅ porous powder on the yeast Candida utilis-as a fungal model-at different phases of growth, i.e., early exponential (6 h), mid-log (11 h), and stationary (17 h) phases. Ca₂Fe₂O₅ inhibited the cell growth in a time-dependent manner. After 120 min incubation, the fungicidal activity of porous powder was observed, i.e., log reduction of 2.81 and 2.58 for 11 and 17 h cultures, respectively, reaching the maximum of 4 log reduction after 7 days. Nevertheless, the 6 h culture of C. utilis showed enhanced resistance to Ca₂Fe₂O₅ with a ≤ 0.4 log reduction during the 7 days exposure. Our results not only showed that Ca₂Fe₂O₅ has the potential to effectively eliminate the C. utilis cell growth but also indicated the importance of the yeast culture physiological state for resistance to Ca₂Fe₂O₅. To the best of our knowledge, this is the first study that evaluated the fungicidal activity of Ca₂Fe₂O₅ porous powder on C. utilis and the impact of the C. utilis phase of growth on the cell susceptibility.

Keywords: Antifungal; Ca-Fe oxide; Candida utilis; Growth kinetics; Yeast; fungi.