Beneficial Effects of lncRNA-UC.360+ shRNA on Diabetic Cardiac Sympathetic Damage via NLRP3 Inflammasome-Induced Pyroptosis in Stellate Ganglion

Liran Shi; Qixing Hu; Lin Li; Runan Yang; Xiumei Xu; Junpei Du; Lifang Zou; Guilin Li; Shuangmei Liu; Guodong Li; Shangdong Liang

doi:10.1021/acsomega.2c03619

Beneficial Effects of lncRNA-UC.360+ shRNA on Diabetic Cardiac Sympathetic Damage via NLRP3 Inflammasome-Induced Pyroptosis in Stellate Ganglion

ACS Omega. 2022 Jul 29;7(31):27714-27721. doi: 10.1021/acsomega.2c03619. eCollection 2022 Aug 9.

Authors

Liran Shi^{1

2

3}, Qixing Hu^{1

3}, Lin Li^{1

3}, Runan Yang^{1

3}, Xiumei Xu^{1

3}, Junpei Du^{1

3}, Lifang Zou^{1

4

3}, Guilin Li^{1

3}, Shuangmei Liu^{1

3}, Guodong Li^{1

3}, Shangdong Liang^{1

3}

Affiliations

¹ Neuropharmacology Laboratory of Physiology Department, Basic Medical School of Nanchang University, Nanchang 330006, PR China.
² The people's hospital of Jiawang of Xuzhou, Xuzhou 221011, China.
³ Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, PR China.
⁴ Department of Haematology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China.

Abstract

Hyperglycemia is one of the common symptoms of diabetes, and it produces excessive reactive oxygen species (ROS). This study investigated whether the long noncoding RNA (lncRNA) UC.360+ is involved in diabetic cardiac autonomic neuropathy (DCAN) mediated by NLRP3 inflammasome-induced pyroptosis in the stellate ganglion (SG). Using a rat type 2 diabetes model, we found that lncRNA UC.360+ short hairpin RNA (shRNA) ameliorated the dyslipidaemia of type 2 diabetic rats and reduced serum adrenaline and ROS production in SG under hyperglycemia. In addition, UC.360+ shRNA also reduced the expression of nuclear factor kappa-B (NF-κB), NLRP3, ASC, caspase-1, interleukin-1β (IL-1β), and IL-18 in the SG of diabetic rats and inhibited the phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK). Therefore, lncRNA-UC.360+ shRNA may modulate the NLRP3 inflammasome/inflammatory pathway in the SG, which in turn alleviates diabetic heart sympathetic nerve damage.