A clinical Pseudomonas juntendi strain with bla IMP-1 carried by an integrative and conjugative element in China

Lin Zheng; Xinfang Zhang; Lingwei Zhu; Gejin Lu; Jiayao Guan; Mingwei Liu; Jie Jing; Shiwen Sun; Ying Wang; Yang Sun; Xue Ji; Bowen Jiang; Jun Chen; Jun Liu; Ping Chen; Xuejun Guo

doi:10.3389/fmicb.2022.929800

A clinical Pseudomonas juntendi strain with bla _IMP-1 carried by an integrative and conjugative element in China

Front Microbiol. 2022 Jul 29:13:929800. doi: 10.3389/fmicb.2022.929800. eCollection 2022.

Authors

Lin Zheng^{1

2}, Xinfang Zhang³, Lingwei Zhu², Gejin Lu², Jiayao Guan², Mingwei Liu^{1

2}, Jie Jing², Shiwen Sun², Ying Wang², Yang Sun², Xue Ji², Bowen Jiang², Jun Chen², Jun Liu², Ping Chen¹, Xuejun Guo²

Affiliations

¹ School of Food and Engineering, Jilin Agricultural University, Changchun, China.
² Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China.
³ China-Japan Union Hospital, Jilin University, Changchun, China.

Abstract

Objective: To precisely determine the species of a carbapenem-resistant Pseudomonas strain 1809276 isolated from the urine of a Chinese patient and analyze its integrative and conjugative element (ICE) 1276 formation mechanism.

Methods: Single-molecule real-time (SMRT) sequencing was carried out on strain 18091276 to obtain the complete chromosome and plasmid (pCN1276) sequences, and average nucleotide identity (ANI) was used for precise species identification. The ICEs in GenBank with the same integrase structure as ICE 1276 were aligned. At the same time, the transfer ability of bla _IMP-1 and the antibiotic sensitivity of Pseudomonas juntendi 18091276 were tested.

Results: This bacterium was P. juntendi, and its drug resistance mechanism is the capture of the accA4' gene cassette by the Tn402-like type 1 integron (IntI1-bla _IMP-1) to form In1886 before its capture by the ΔTn4662a-carrying ICE 1276. The acquisition of bla _IMP-1 confers carbapenem resistance to P. juntendi 18091276.

Conclusion: The formation of bla _IMP-1-carrying ICE 1276, its further integration into the chromosomes, and transposition and recombination of other elements promote bacterial gene accumulation and transmission.

Keywords: Pseudomonas juntendi; blaIMP−1; carbapenem-resistant; integrative and conjugative; reorganization.