Evaluation of cancer stem cells markers expression in HCC trough real-time polymerase chain reaction

Methods Cell Biol. 2022:171:23-32. doi: 10.1016/bs.mcb.2022.04.003. Epub 2022 Jul 14.

Abstract

Quantitative real-time polymerase chain reaction (qRT-PCR) flexibility, robustness and reproducibility have rapidly extended the scope of the method. Cancer stem cells are gaining increasing importance since their role in cancer initiation, treatment resistance and recurrence give rise to a wide range of potential diagnostic and therapeutic applications. The expression of several characteristic markers is proven a reliable method to assess stem-like-phenotype of cancer cells. Here, we provided a thorough protocol for the study of cancer stem cells in hepatocellular carcinoma mouse models and cell cultures using qRT-PCR.

Keywords: CD133; CD44; CD47; Cancer stem cells; EpCAM; Hepatocellular carcinoma; OCT4; Quantitative real-time polymerase chain reaction.

MeSH terms

  • AC133 Antigen / genetics
  • AC133 Antigen / metabolism
  • Animals
  • Carcinoma, Hepatocellular* / genetics
  • Carcinoma, Hepatocellular* / metabolism
  • Carcinoma, Hepatocellular* / pathology
  • Liver Neoplasms* / genetics
  • Liver Neoplasms* / pathology
  • Mice
  • Neoplastic Stem Cells / pathology
  • Real-Time Polymerase Chain Reaction
  • Reproducibility of Results

Substances

  • AC133 Antigen