Genetic data of 22 autosomal STR loci in Khyber-Pakhtunkhwa, Balochistan and Gilgit Baltistan population of Pakistan using PowerPlex® fusion system

Muhammad Shafique; Namra Sajid; Yasir Aziz; Muhammad Afzal; Muhammad Shahzad; Abida Shehzadi; Saliha Bashir; Shakeela Daud; Ahmad Ali Shahid

doi:10.1016/j.legalmed.2022.102129

Genetic data of 22 autosomal STR loci in Khyber-Pakhtunkhwa, Balochistan and Gilgit Baltistan population of Pakistan using PowerPlex® fusion system

Leg Med (Tokyo). 2022 Nov:59:102129. doi: 10.1016/j.legalmed.2022.102129. Epub 2022 Aug 3.

Authors

Muhammad Shafique¹, Namra Sajid², Yasir Aziz³, Muhammad Afzal⁴, Muhammad Shahzad⁵, Abida Shehzadi⁶, Saliha Bashir⁷, Shakeela Daud⁸, Ahmad Ali Shahid⁹

Affiliations

¹ Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab Lahore 53700, Pakistan. Electronic address: shafique@cemb.edu.pk.
² Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab Lahore 53700, Pakistan. Electronic address: namraminhas65@gmail.com.
³ Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab Lahore 53700, Pakistan. Electronic address: abdyasir007@gmail.com.
⁴ Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab Lahore 53700, Pakistan. Electronic address: afzal9737@gmail.com.
⁵ Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab Lahore 53700, Pakistan. Electronic address: shahzad.camb@pu.edu.pk.
⁶ Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab Lahore 53700, Pakistan. Electronic address: abida@cemb.edu.pk.
⁷ Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab Lahore 53700, Pakistan. Electronic address: saliha18bashir@gmail.com.
⁸ Faculty of Life Sciences and Informatics, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta (BAUITEMS), Pakistan. Electronic address: Shakeela.daud@buitms.edu.pk.
⁹ Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab Lahore 53700, Pakistan. Electronic address: aali@cemb.edu.pk.

PMID: 35944342
DOI: 10.1016/j.legalmed.2022.102129

Abstract

In this study we have provided forensic genetic data of 22 autosomal STRs for Pakhtun, Balochi and Balti population of Pakistan in total of 601 samples. Among these loci, Penta E was found the most discriminatory in all groups and allele 15 was observed most frequent at D22S1045 in Balti whereas in other two populations allele 8 was more common at TPOX. The combined power of discrimination, combined power of exclusion and the combined matching probability was calculated as 0.999999999999999999999999998385, 0.999999988089728 and 1.615 × 10^-27 respectively. Based on population differentiation test, significant differences were observed when compared with other populations however, phylogenetic analysis revealed close genetic associations among Pakistani Populations.

Keywords: Autosomal STRs; Balochistan; Gilgit Baltistan; Khyber-Pakhtunkhwa; PowerPlex fusion.

MeSH terms

Gene Frequency / genetics
Genetic Loci
Genetics, Population*
Humans
Microsatellite Repeats* / genetics
Pakistan
Phylogeny