A novel umami electrochemical biosensor based on AuNPs@ZIF-8/Ti3C2 MXene immobilized T1R1-VFT

Jing Liu; Ninglong Zhang; Jiansen Li; Mingyang Li; Guangxian Wang; Wenli Wang; Yuxia Fan; Shui Jiang; Gaole Chen; Yin Zhang; Xia Sun; Yuan Liu

doi:10.1016/j.foodchem.2022.133838

A novel umami electrochemical biosensor based on AuNPs@ZIF-8/Ti₃C₂ MXene immobilized T1R1-VFT

Food Chem. 2022 Dec 15:397:133838. doi: 10.1016/j.foodchem.2022.133838. Epub 2022 Jul 30.

Authors

Jing Liu¹, Ninglong Zhang², Jiansen Li¹, Mingyang Li², Guangxian Wang¹, Wenli Wang², Yuxia Fan², Shui Jiang², Gaole Chen², Yin Zhang³, Xia Sun⁴, Yuan Liu⁵

Affiliations

¹ School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, Shandong Province, China.
² Department of Food Science & Technology, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
³ Key Laboratory of Meat Processing of Sichuan, Chengdu University, Chengdu 610106, China.
⁴ School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, Shandong Province, China. Electronic address: sunxia2151@sina.com.
⁵ Department of Food Science & Technology, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: y_liu@sjtu.edu.cn.

PMID: 35944333
DOI: 10.1016/j.foodchem.2022.133838

Abstract

The bioelectronic tongues based on taste receptors have been emerging with human-like taste perception. However, the practical applications of the receptor-based biosensors were restricted by their narrow and low dynamic ranges. Here, a novel immobilization strategy based on AuNPs@ZIF-8/Ti₃C₂ MXene was developed to immobilize the umami ligand binding domain (T1R1-VFT), to fabricate an umami biosensor for umami substances detection. Through the synergic effect of AuNPs@ZIF-8 and Ti₃C₂ MXene, the capacity to load T1R1-VFT was effectively increased, and the response signal was also amplified by approximately 3 times. The proposed biosensor showed an ultrawide dynamic range of 10^-11-10^-3 M, and a high upper limit of detection, which was closer to the human taste threshold and suitable for detecting foods rich in umami substances. Additionally, the biosensor was successfully applied to detect real samples and analyze the synergistic effects of binary umami substances.

Keywords: AuNPs@ZIF-8/Ti(3)C(2) MXene; Immobilization; T1R1 Venus flytrap (VFT); Umami electrochemical biosensor.

MeSH terms

Biosensing Techniques*
Gold
Humans
Metal Nanoparticles*
Receptors, G-Protein-Coupled / metabolism
Taste
Titanium / chemistry

Substances

Receptors, G-Protein-Coupled
Gold
Titanium