Association between PAX8AS1 (rs4848320 C > T, rs1110839 G > T, and rs6726151 T > G) and MEG3 (rs7158663) gene polymorphisms and non-Hodgkin lymphoma risk

Samaneh Mirzazadeh; Hosna Sarani; Alireza Nakhaee; Seyed-Mehdi Hashemi; Mohsen Taheri; Mohammad Hashemi; Gholamreza Bahari

doi:10.1080/15257770.2022.2104870

Association between PAX8AS1 (rs4848320 C > T, rs1110839 G > T, and rs6726151 T > G) and MEG3 (rs7158663) gene polymorphisms and non-Hodgkin lymphoma risk

Nucleosides Nucleotides Nucleic Acids. 2022;41(11):1174-1186. doi: 10.1080/15257770.2022.2104870. Epub 2022 Aug 8.

Authors

Samaneh Mirzazadeh¹, Hosna Sarani^{2

3}, Alireza Nakhaee¹, Seyed-Mehdi Hashemi⁴, Mohsen Taheri³, Mohammad Hashemi¹, Gholamreza Bahari^{1

2}

Affiliations

¹ Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
² Children and Adolescent Health Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran.
³ Genetics of Non-Communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
⁴ Department of Internal Medicine, Clinical Immunology Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.

PMID: 35938744
DOI: 10.1080/15257770.2022.2104870

Abstract

Long non-coding RNA (lncRNA) PAX8 antisense RNA 1 (PAX8AS1) and Maternal-expressed gene 3 (MEG3) contribute to the pathogenesis of various malignancies including non-Hodgkin lymphoma (NHL). In this study, we aimed to examine the possible association of polymorphisms of PAX8 and MEG3 and the risk NHL. A total of 175 patients and 175 healthy subjects were genotyped by PCR-RFLP and Tetra-Arms PCR assays. Results demonstrated rs4848320 C > T and rs6726151 T > G of PAX8AS1 and rs7158663 of MEG3 play a potential role in the susceptibility of NHL and PAX8AS1 rs1110839 T > G variant was associated with decreased risk of NHL. Haplotype analysis of rs1110839, rs4848320, and rs6726151 demonstrated GCG haplotype is associated with increased risk of lymphoma and TTG, TTT, and GTT haplotypes are related to decreased lymphoma susceptibility.

Keywords: MEG3; Non-Hodgkin lymphoma; PAX8; long non-coding RNAs; polymorphism.

MeSH terms

Case-Control Studies
Genetic Predisposition to Disease
Humans
Lymphoma, Non-Hodgkin* / genetics
Polymorphism, Single Nucleotide
RNA, Antisense
RNA, Long Noncoding* / genetics

Substances

RNA, Antisense
RNA, Long Noncoding
MEG3 non-coding RNA, human