Comparative transcriptome analysis of Lupinus polyphyllus Lindl. provides a rich molecular resource for research on coloration mechanism

Zhu Gao; Jipeng Mao; Lu Chen; Xiaoling Wang; Lu Zhang

doi:10.7717/peerj.13836

Comparative transcriptome analysis of Lupinus polyphyllus Lindl. provides a rich molecular resource for research on coloration mechanism

PeerJ. 2022 Aug 2:10:e13836. doi: 10.7717/peerj.13836. eCollection 2022.

Authors

Zhu Gao^{1

2}, Jipeng Mao², Lu Chen³, Xiaoling Wang², Lu Zhang¹

Affiliations

¹ College of Forestry, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
² Institute of Biological Resources, jiangxi Academy of Sciences, Nanchang, Jiangxi, China.
³ Jinggangshan Institute of Biotechnology, Nanchang, Jiangxi, China.

Abstract

Lupinus polyphyllus is rich in color, making it a well-known horticultural ornamental plant. However, little is known about the genes related to anthocyanin and carotenoid biosynthesis in L. polyphyllus. In this study, transcriptome sequencing was performed on eight different colors of L. polyphyllus. A total of 1.13 billion clean reads were obtained and assembled into 89,124 unigenes, which were then aligned with six databases, resulting in the identification of 54,823 annotated unigenes. Among these unigenes, 76 and 101 were involved in the biosynthetic pathway of carotenoids and anthocyanins, respectively. In addition, 505 transcription factors were revealed, which belonged to the MYB, R2R3-MYB, NAC, bHLH, and WD40 families. A total of 6,700 differentially expressed genes (DEGs) were obtained by comparative transcriptome analysis. Among them, 17 candidate unigenes (four carotenoid genes, seven anthocyanin genes, and six TFs) were specifically up-regulated for one or more colors of L. polyphyllus. Eight representative candidate unigenes were analyzed by qRT-PCR. The findings enrich the transcriptome database of lupine, and provide a rich molecular resource for research on the coloration mechanism of L. polyphyllus.

Keywords: Anthocyanin; Carotenoid; Genes; Lupinus polyphyllus; Transcriptome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anthocyanins / genetics
Carotenoids
Gene Expression Profiling / methods
Humans
Lupinus* / genetics
Transcriptome* / genetics

Substances

Anthocyanins
Carotenoids

Grants and funding

This work was supported by the Key Scientific and Technological Achievements Transfer and Transformation Plan Project of Jiangxi Province: ‘Application and demonstration of ornamental lupine in landscape’ (grant number 20161ACI90012), and the Science and Technology Innovation High-end Talent Project of Jiangxi Province (grant number jxsq20191010). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.