One-pot fabrication of nanozyme with 2D/1D heterostructure by in-situ growing MoS2 nanosheets onto single-walled carbon nanotubes with enhanced catalysis for colorimetric detection of glutathione

Abstract

A nanozyme with 2D/1D heterostructure has been fabricated by the in-situ growth of molybdenum disulfide nanosheets (MoS₂ NSs) onto single-walled carbon nanotubes (SWCNTs). It was discovered that the so-obtained SWCNTs@MoS₂ nanozyme could exhibit greatly improved peroxidase-like catalysis, due to that the formed 2D/1D interfacial coupling in the heterostructure might provide more active sites and exhibit enhanced charge transferring during the catalytic reactions, as confirmed by the X-ray photoelectron spectroscopy, photoluminescence, electrochemical impedance spectra and radical capturing experiments. Furthermore, the catalysis of the developed nanozyme could be selectively inhibited by glutathione (GSH) through the competitive consumption of hydroxyl radicals with enzyme substrate in the catalytic reaction system. A SWCNTs@MoS₂ catalysis-based colorimetric strategy was further proposed for the quantitative analysis of GSH with the concentrations linearly ranging from 0.01 to 1000.0 μM. Besides, the feasibility of the developed colorimetric method was evaluated by monitoring GSH separately in the extractions from hela cells and human serum, promising the extensive applications for monitoring various biological species like GSH in the clinical laboratory. Importantly, such a fabrication route for nanozyme with 2D/1D heterostructure may pave the way towards the wide applications for designing various nanzymes with improved catalysis.

Keywords: 2D/1D heterostructure; Glutathione; MoS(2) nanosheets; Peroxidase mimic; Single-walled carbon nanotubes.