The in vitro replication phenotype of hepatitis B virus (HBV) splice variant Sp1

V Sozzi; L McCoullough; H Mason; M Littlejohn; P A Revill

doi:10.1016/j.virol.2022.07.005

The in vitro replication phenotype of hepatitis B virus (HBV) splice variant Sp1

Virology. 2022 Sep:574:65-70. doi: 10.1016/j.virol.2022.07.005. Epub 2022 Jul 21.

Authors

V Sozzi¹, L McCoullough¹, H Mason¹, M Littlejohn¹, P A Revill²

Affiliations

¹ Victorian Infectious Diseases Reference Laboratory, Royal Melbourne Hospital at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
² Victorian Infectious Diseases Reference Laboratory, Royal Melbourne Hospital at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia. Electronic address: Peter.revill@mh.org.au.

PMID: 35930906
DOI: 10.1016/j.virol.2022.07.005

Abstract

Although not critical for hepatitis B virus (HBV) replication, splicing of HBV pre-genomic RNA generates multiple HBV splice variants, some of which have been shown to impact replication of the genome-length HBV on which they rely for their replication. To date, all replication studies of splice variants have utilised truncated RNA or over-expression constructs, and studies utilising constructs that produce authentic splice derived HBV RNA are lacking. Here we utilise a greater than genome length model to interrogate the complete replication phenotype of HBV splice variant Sp1, and investigate mechanisms by which it negatively impacts genome-length HBV replication.

Keywords: Core; HBx; Hepatitis B virus; Precore; Replication; SP1; Splicing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Hepatitis B virus* / genetics
Hepatitis B*
Humans
Mutation
Phenotype
RNA
Sp1 Transcription Factor / genetics
Virus Replication / genetics

Substances

Sp1 Transcription Factor
SP1 protein, human
RNA