Diverse and distinct bacterial community involved in a full-scale A/O1/H/O2 combination of bioreactors with simultaneous decarbonation and denitrogenation of coking wastewater

Abstract

Taking into account difficulties in exhaustive simultaneous decarbonation and denitrogenation in biological treatment of coking wastewater (CWW), a novel full-scale CWW biological treatment sequentially combining anaerobic, aerobic, hydrolytic, and aerobic reactors (A/O1/H/O2) was designed performing excellent removal of carbon-containing pollutants in the bioreactors A and O1, while the nitrogen-containing compounds in the bioreactors H and O2. To provide an effective tool for the CWW treatment monitoring and control, the succession of microbial community in this unique toxic CWW habitat should be established and characterized in detail. The results of 16S rRNA genes revealed Acidobacteria dominating in the unique CWW habitat. The dominant groups in bioreactors A and O1 include Proteobacteria, Firmicutes, and Acidobacteria, while Proteobacteria, Acidobacteria, Nitrospirae, and Planctomycetes dominate in reactors H and O2. The genera of Rhodoplanes, Bacillus, and Leucobacter are rich in genes responsible for the xenobiotics biodegradation and metabolism pathway. The Mantel test and PCA results showed the microbial communities of A/O1/H/O2 sequence correlating strongly with SRT, and COD load and removal. The co-occurrence network analysis indicated decarbonation and denitrogenation driven by two network modules having the keystone taxa belonging to the Comamonadaceae and Hyphomicrobiaceae families. The results significantly expanded the knowledge on the diversity, structure, and function of the CWW active sludge differentiating the relationships between bacterial communities and environmental variables in CWW treatment.

Keywords: 16S rDNA; Coking wastewater; Diversity; Functional prediction; Microbiology.