Up-conversion nanoparticles (UCNPs) of sodium yttrium fluoride with ytterbium and erbium ions as sensitizer and activator (β-NaYF4/Yb3+/Er3+) have been synthesised by a solvothermal method. The synthesised particles were found to be highly uniform in size (~50 nm) and of hexagonal crystal phase producing strong up-conversion luminescence dominated in the green wavelength region. During the synthesis, photoluminescence properties of the reaction mixture were monitored at regular intervals to ensure the required particle size distribution and luminescence efficiency. The hydrophobic particles thus obtained were modified by coating with silica, yielding particles that were stable in aqueous media. The silica coated UCNPs were further modified with maleimide-polyethylene glycol-silane (mal-PEG-silane) to provide thiol reactive surface groups. The silanized, maleimide-bearing UCNPs were effective for conjugating to reductively-cleaved half antibodies against ofloxacin, a veterinary antibiotic, to produce photoluminescent nanobiosensors for its detection and quantification. The speed and minimum detection concentration (~10 nM) that we report for a competitive assay of ofloxacin in this study is promising for developing sensors for this and other biomolecules.
Keywords: Competitive assay; Detection; Ofloxacin; Photoluminescence; Up-conversion nanoparticles.
Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.