Evaluation of the effects of four types of tea on the activity of cytochrome P450 enzymes with a probe cocktail and HPLC-MS/MS

Xueyan Wang; Yanping Liu; Liming Ye; Ying Wei; Yingqiang Fu; Yu Chen; Linchuan Liao

doi:10.21037/atm-21-5490

Evaluation of the effects of four types of tea on the activity of cytochrome P450 enzymes with a probe cocktail and HPLC-MS/MS

Ann Transl Med. 2022 May;10(9):504. doi: 10.21037/atm-21-5490.

Authors

Xueyan Wang¹, Yanping Liu², Liming Ye², Ying Wei³, Yingqiang Fu³, Yu Chen⁴, Linchuan Liao³

Affiliations

¹ Department of Forensic Analytical Toxicology, West China School of Basic Medical Sciences & Forensic Medicine, and West China Hospital, Sichuan University, Chengdu, China.
² West China School of Pharmacy, Sichuan University, Chengdu, China.
³ Department of Forensic Analytical Toxicology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, China.
⁴ Department of Pharmacy, West China Hospital of Sichuan University, Chengdu, China.

Abstract

Background: Tea, the world's second most popular drink, is an essential part of some people's lives. Thus, this study aimed to explore potential tea-drug interactions with a view to promoting the rational administration of drugs.

Methods: A specific and sensitive approach involving high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and a probe cocktail was established and validated to evaluate the inhibitory effects of four teas on five cytochrome P450 (CYP450) enzymes in rats. Metoprolol tartrate (MT), omeprazole (OMP), phenacetin (PNT), tolbutamide (TOL), and testosterone (T) were selected as the probe drugs for CYP2D6, CYP2C19, CYP1A2, CYP2C6, and CYP3A1/2, respectively, and were simultaneously quantified in the multiple reaction monitoring (MRM) mode with positive electrospray ionization (ESI+) in a single 12-min run.

Results: The extraction recoveries, matrix effect values, as well as intra/interday accuracy and precision met the determination standards. CYP1A2 and CYP2C6 were strongly inhibited by green tea, and CYP2C6 was also strongly inhibited by Pu'er tea. Ti Kuan Yin tea had a weak inhibitory effect, and black tea had only a slight inhibitory effect, on CYP1A2. Furthermore, the four types of tea did not have significantly altered the activity of CYP2D6, CYP2C19, and CYP3A1/2 in vitro.

Conclusions: The method used in the present study was successfully applied to assess the inhibitory effects of aqueous extracts of four types of tea on CYP450 isoforms in vitro. The results suggest that different types of tea have different effects on drug metabolism.

Keywords: High-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS); aqueous extracts of tea; cytochrome P450 enzymes; probe cocktail; tea-drug interactions.