Microbial mechanisms of refractory organics degradation in old landfill leachate by a combined process of UASB-A/O-USSB

Abstract

A combined process of anaerobic digestion (UASB), shortcut nitrification-denitrification (A/O), and semi-anoxic co-metabolism (operated by an up-flow semi-anoxic sludge bed; USSB) was constructed for the treatment of old landfill leachate (>10 years). The performance and mechanism of refractory organics degradation by the combined process (UASB-A/O-USSB) were investigated. The results showed that the semi-anoxic co-metabolism contributes 57 % of the totally degraded refractory organics. Specific microorganisms and their corresponding metabolic functions drive the degradation of refractory organics in each unit of the UASB-A/O-USSB process. In detail, organics with simple molecular structures were preferentially degraded by anaerobic digestion and shortcut denitrification, whereas those with complex structures were subsequently degraded in the oxic tanks and USSB reactor by shortcut nitrification and semi-anoxic co-metabolism. The structural equation model showed that the combined process of shortcut nitrification and semi-anoxic co-metabolism had a better effect on the degradation of recalcitrant organics than the single process. These findings provide information on how refractory organics are metabolically degraded in a combined process.

Keywords: Microbial degradation mechanisms; Old landfill leachate; Refractory organics; Semi-anoxic co-metabolism; Shortcut nitrification-denitrification.