Complete sequence of Cynanchum rostellatum (Apocynaceae: Asclepiadoideae) chloroplast genome and its phylogenetic analysis

Lixin Pei; Shengnan Shu; Baoyu Ji; Ning Cui

doi:10.1080/23802359.2022.2102444

Complete sequence of Cynanchum rostellatum (Apocynaceae: Asclepiadoideae) chloroplast genome and its phylogenetic analysis

Mitochondrial DNA B Resour. 2022 Jul 29;7(7):1395-1397. doi: 10.1080/23802359.2022.2102444. eCollection 2022.

Authors

Lixin Pei¹, Shengnan Shu^{1

2}, Baoyu Ji¹, Ning Cui³

Affiliations

¹ College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, China.
² School of Pharmacy and Chemical Engineering, Zhengzhou University of Industry Technology, Zhengzhou, China.
³ Central Laboratory, Shandong Academy of Chinese Medicine, Ji'nan, China.

Abstract

Cynanchum rostellatum (Turcz.) Liede and Khanum 2016 is a perennial herbaceous twining vine that is widely distributed in Japan, South Korea, the United States of America, and China. In this study, the complete chloroplast (cp) genome of C. rostellatum was sequenced using the Illumina platform and assembled for the first time. This plastome has a circular structure with a length of 160,641 bp. The GC content of the plastome was 37.82%. The cp genome contained 113 unique genes, including 79 protein-coding, 30 transfer RNA, and four ribosomal RNA genes. Phylogenetic analysis based on the complete cp genome sequences of the Asclepiadoideae subfamily showed that C. rostellatum was closely related to C. bungei in the genus Cynanchum. These results provide useful information for both phylogenetic research and the utilization of C. rostellatum.

Keywords: Cynanchum rostellatum; chloroplast genome; phylogeny.

Grants and funding

This work was supported by the Shandong Province Traditional Chinese Medicine Science and Technology development Program [2019–0650], Research Incubation Fund Project of Shandong Academy of Chinese Medicine [2021SACM-3], Medical and Health Science and Technology Development Project of Shandong Province [202102041135], “National Survey of Traditional Chinese Medicine Resources”, a special subsidy program for United States Public Health Service of traditional Chinese medicine in 2017 [caishe[2017] No. 66] and “National Survey of Traditional Chinese Medicine Resources”, a special subsidy program for United States Public Health Service of traditional Chinese medicine in 2018 [caishe[2017] No.183].