Phenolic composition and neuroprotective effects of the ethyl-acetate fraction from Inonotus sanghuang against H2O2-induced apoptotic cell death of primary cortical neuronal cells

Kun Liu; Cuilian Qi; Yihang Liu; Yaping Huai; Huagang Hu; Xuan Xiao; Junpeng Wang

doi:10.1007/s10068-022-01107-x

Phenolic composition and neuroprotective effects of the ethyl-acetate fraction from Inonotus sanghuang against H₂O₂-induced apoptotic cell death of primary cortical neuronal cells

Food Sci Biotechnol. 2022 Jun 24;31(9):1213-1223. doi: 10.1007/s10068-022-01107-x. eCollection 2022 Aug.

Authors

Kun Liu^{1

2}, Cuilian Qi¹, Yihang Liu³, Yaping Huai⁴, Huagang Hu⁵, Xuan Xiao⁵, Junpeng Wang⁶

Affiliations

¹ College of Biology Science and Engineering, Hebei University of Economics and Business, Shijiazhuang, 050061 Hebei China.
² Department of Mathematics and Statistics, Hebei University of Economics and Business, Shijiazhuang, 050061 Hebei China.
³ Department of Computer Science, Wenzhou-Kean University, Wenzhou, 325000 Zhejiang China.
⁴ Department of Rehabilitation, Shenzhen Longhua District Central Hospital, Shenzhen, 518000 Guangdong China.
⁵ Chronic Disease Rehabilitation Laboratory of Integrated Traditional Chinese and Western Medicine, Beijing Xiaotangshan Hospital, Beijing, 100081 China.
⁶ Institute of Infection and Immunity of Huaihe Hospital, Henan University, Kaifeng, 475000 Henan China.

Abstract

The study aimed to characterize phenolic compounds of the Inonotus sanghuang's ethyl-acetate fraction (EAF) and assess the neuroprotective effect of EAF using the H₂O₂-treated primary cortical neuronal cells (PCNC) model. Using HPLC-ECD, 5 phenolics were identified and quantified from EAF. H₂O₂-treated PCNC experiments in vitro showed that pretreatment with EAF increased the GSH-PX and SOD activities and reduced the NO, MDA, and Aβ contents. Furthermore, EAF suppressed the production of IL-1β, IFN-γ, IL-6, and TNF-α in H₂O₂-treated PCNC. Other mechanisms found that EAF reduced Bax, caspase 9, and caspase 3 expressions at the mRNA and protein levels while increasing Bcl-2 expression at the mRNA and protein levels. These results showed that EAF could serve as potential agents for anti-NDD.

Supplementary information: The online version contains supplementary material available at 10.1007/s10068-022-01107-x.

Keywords: Anti-apoptosis; Anti-inflammatory; Antioxidation; Inonotus sanghuang; Neuroprotective effect.