A practical method for gas changing time estimation using a simple gas-liquid mass transfer model

J Microbiol Methods. 2022 Sep:200:106544. doi: 10.1016/j.mimet.2022.106544. Epub 2022 Jul 30.

Abstract

The present work explains a practical and simple method to calculate the gas changing time of anaerobic systems. It is substantiated under the physics of gas-liquid transfer theory and allows researchers to obtain an approximate value of gas changing time with few measurements of the gas composition in the outlet of the reactor. The only analytical equipment required is a gas analyzer, and calculations can be done using a spreadsheet. Along with the validation of the model, a short guide for its application in the laboratory is introduced. The model fit the experimental data with less than 1% error in the composition of the out-gas when no carbon dioxide is involved. This method will allow savings in valuable resources such as time and gases while providing greater comprehension of the characteristics of the gas-liquid transfer of the studied system.

Keywords: Anaerobic fermentation; Gas changing time; Oxygen purging.