Oligodendrocytes in the periaqueductal gray matter and the corpus callosum in adult male and female domestic sheep

Agata Wawrzyniak; Krzysztof Balawender; Roman Lalak; Rafał Staszkiewicz; Dariusz Boroń; Beniamin Oskar Grabarek

doi:10.1016/j.brainres.2022.148036

Oligodendrocytes in the periaqueductal gray matter and the corpus callosum in adult male and female domestic sheep

Brain Res. 2022 Oct 1:1792:148036. doi: 10.1016/j.brainres.2022.148036. Epub 2022 Jul 29.

Authors

Agata Wawrzyniak¹, Krzysztof Balawender², Roman Lalak³, Rafał Staszkiewicz⁴, Dariusz Boroń⁵, Beniamin Oskar Grabarek⁶

Affiliations

¹ Department of Morphological Sciences, College of Medical Sciences, Institute of Medical Sciences, University of Rzeszów, Rzeszów, Poland. Electronic address: a17041962@gmail.com.
² Department of Morphological Sciences, College of Medical Sciences, Institute of Medical Sciences, University of Rzeszów, Rzeszów, Poland. Electronic address: krzysztofbalawender2@gmail.com.
³ Department of Animal Anatomy and Histology, University of Life Sciences in Lublin, Lublin, Poland. Electronic address: roman.lalak@up.lublin.pl.
⁴ 5th Military Clinical Hospital with the SP ZOZ Polyclinic in Krakow, 30-901 Krakow, Poland; Department of Histology, Cytophysiology and Embryology, Faculty of Medicine in Zabrze, University of Technology, Academy of Silesia in Katowice, 41-800 Zabrze, Poland. Electronic address: rafalstaszkiewicz830@gmail.com.
⁵ Department of Histology, Cytophysiology and Embryology, Faculty of Medicine in Zabrze, University of Technology, Academy of Silesia in Katowice, 41-800 Zabrze, Poland; Department of Gynaecology and Obsterics, Faculty of Medicine in Zabrze, University of Technology, Academy of Silesia in Katowice, 41-800 Zabrze, Poland. Electronic address: dariusz@boron.pl.
⁶ Department of Histology, Cytophysiology and Embryology, Faculty of Medicine in Zabrze, University of Technology, Academy of Silesia in Katowice, 41-800 Zabrze, Poland; Department of Gynaecology and Obsterics, Faculty of Medicine in Zabrze, University of Technology, Academy of Silesia in Katowice, 41-800 Zabrze, Poland; GynCentrum, Laboratory of Molecular Biology and Virology, 40-851 Katowice, Poland. Electronic address: bgrabarek7@gmail.com.

PMID: 35908588
DOI: 10.1016/j.brainres.2022.148036

Abstract

There is little information about oligodendrocytes (OLGs) in the Periaqueductal Gray matter (PAG). The literature has not provided data on the number, morphology, or quantification of the expression of the OLG protein yet. Myelin Basic Protein (MBP) in this region of the Central Nervous system (CNS). The study aimed was to perform a comparative analysis: the location and morphology of OLGs, the cellular and regional distribution of iron, and the number of OLGs in PAG and corpus callosum (CC) of adult 16 male and 16 female sheep. To determine the location of the OLG of PAG and CC, the method of impregnation of the neuroglia with silver salts was applied. In turn, the Nissl method was used to determine the location of the brain structure and to analyse the number of OLG. The performed analysis showed that PAG, OLGs are located singly or in pairs in blood vessels and neurons, while in CC they are arranged in characteristic rows and accompany both nerve fibres and blood vessels. Immunofluorescent staining for the presence of MBP confirmed the location of OLGs in male and female sheep. Morphometric analysis showed the importance of these glial cells in OLG-myelin fibres, correlation in adults regardless of sex even after the creation of the completion of myelin. The results obtained indicate that the functions of OLGs are not only confined to myelination in young individuals, but also play a crucial role in the brain of adults. Our observations seem to be useful to better understanding OLGs biology.

Keywords: Glial cells; Oligodendrocyte; Periaqueductal Gray matter; Sheep.

MeSH terms

Animals
Corpus Callosum* / metabolism
Female
Male
Myelin Basic Protein / metabolism
Myelin Sheath / metabolism
Oligodendroglia / metabolism
Periaqueductal Gray / metabolism
Sheep
Sheep, Domestic* / metabolism

Substances

Myelin Basic Protein