Surface pre-reacted glass-ionomer eluate protects gingival epithelium from penetration by lipopolysaccharides and peptidoglycans via transcription factor EB pathway

Hiroki Takeuchi; Yuta Kato; Naoko Sasaki; Keita Tanigaki; Shunsuke Yamaga; Ena Mita; Masae Kuboniwa; Michiya Matsusaki; Atsuo Amano

doi:10.1371/journal.pone.0271192

Surface pre-reacted glass-ionomer eluate protects gingival epithelium from penetration by lipopolysaccharides and peptidoglycans via transcription factor EB pathway

PLoS One. 2022 Jul 27;17(7):e0271192. doi: 10.1371/journal.pone.0271192. eCollection 2022.

Authors

Hiroki Takeuchi¹, Yuta Kato¹, Naoko Sasaki², Keita Tanigaki¹, Shunsuke Yamaga¹, Ena Mita¹, Masae Kuboniwa¹, Michiya Matsusaki^{2

3}, Atsuo Amano¹

Affiliations

¹ Department of Preventive Dentistry, Graduate School of Dentistry, Osaka University, Suita, Osaka, Japan.
² Joint Research Laboratory (TOPPAN) for Advanced Cell Regulatory Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.
³ Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.

Abstract

Surface pre-reacted glass-ionomer (S-PRG) filler, produced by PRG technology for use with various dental materials, is bioactive and known to release ions from a glass-ionomer phase. We previously reported that coxsackievirus and adenovirus receptor (CXADR), a tight junction associated protein, was located in the epithelial barrier of gingival epithelium. In the present study, the tissue protective effects of an S-PRG eluate prepared with S-PRG filler were investigated using a three-dimensional human gingival epithelial tissue model. The results showed that the S-PRG eluate specifically induced CXADR expression at the transcriptional level of messenger RNA as well as the protein level, and also nuclear translocation of transcription factor EB (TFEB) in gingival epithelial cells. Furthermore, shigyakusan, a TFEB inhibitor, canceled induction of the CXADR protein by the S-PRG eluate. Additionally, gingival epithelial permeation by 40-kDa dextran, lipopolysaccharide, and peptidoglycan in the 3D-tissue models was prevented by the eluate, with those effects abrogated by knockdown of CXADR. These findings suggest that S-PRG eluate increases CXADR expression via the TFEB pathway, thus inhibiting penetration of bacterial virulence factors into subepithelial tissues.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Epithelium
Glass Ionomer Cements* / pharmacology
Humans
Lipopolysaccharides* / pharmacology
Peptidoglycan
Transcription Factors

Substances

Glass Ionomer Cements
Lipopolysaccharides
Peptidoglycan
Transcription Factors

Grants and funding

This research was supported by JSPS KAKENHI Scientific Research (C) 19K10085 (to H. T.) from the Japan Society for the Promotion of Science, and the Fund for Scientific Promotion J190801017 (to A.A) of SHOFU Inc. (Kyoto, Japan). The funders had no role in study design, data collection, decision to publish, or preparation of the manuscript. 1) JSPS website: https://www.jsps.go.jp/english/index.html 2) Shofu website: https://www.shofu.com/global/.