The Protective Effect of Lycium Ruthenicum Murr Anthocyanins in Cr (VI)-Induced Mitophagy in DF-1 Cells

Shuhua Guo; Mengzhu Qi; Hongyan Li; Yukun Cui; Changxi Qi; Guodong Cheng; Meiyun Lv; Pimiao Zheng; Jianzhu Liu

doi:10.3390/life12081115

The Protective Effect of Lycium Ruthenicum Murr Anthocyanins in Cr (VI)-Induced Mitophagy in DF-1 Cells

Life (Basel). 2022 Jul 25;12(8):1115. doi: 10.3390/life12081115.

Authors

Shuhua Guo¹, Mengzhu Qi², Hongyan Li³, Yukun Cui², Changxi Qi², Guodong Cheng¹, Meiyun Lv¹, Pimiao Zheng², Jianzhu Liu^{1

2}

Affiliations

¹ College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
² Research Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai'an 271018, China.
³ Central Hospital of Tai'an City, Tai'an 271018, China.

Abstract

Cr (VI) is an extremely toxic environment and professional pollutant that seriously damages mitochondrial dysfunction when it enters a cell. Anthocyanins possess anti-oxidant, antiaging, and antifatigue properties. The regulatory effect of Lycium ruthenicum Murr anthocyanin (LRMA) on Cr (VI)-induced mitophagy in DF-1 cells was determined. The experimental design was divided into blank group, groups subjected to Cr (VI) and Cr (VI), and LRMA co-treatment groups. Cell viability was determined by the CCK-8 assay. Mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) were assessed by flow cytometry and immunofluorescence. Mitophagy was monitored by ELISA and Western blot. Data showed that Cr (VI) caused the overexpression of autophagy-related proteins (LC3, Beclin-1) and reduced the expressions of autophagy protein p62 and TOMM20. Compared with the Cr (VI) group, the LRMA group showed considerably decreased mitochondrial damage and mitophagy. LRMA decreased the mitochondrial protein expression of PINK1 and Parkin's transfer from the cytoplasm to mitochondria. LRMA may confer protective effects by reducing PINK1/Parkin-mediated mitophagy in Cr (VI)-induced DF-1 cell models.

Keywords: DF-1 Cells; PINK1/Parkin; anthocyanin; chromium (VI); mitophagy.

Grants and funding

This project was supported by the Key Research and Development Program of Shandong Province (Important Science and Technology Innovation Project) (2019JZZY010735), the National Nature Science Foundation of China (No. 31872535), and the Shandong Natural Science Foundation of China (ZR2018MC027).