A New Biorecognition-Element-Free IDμE Sensor for the Identification and Quantification of E. coli

Yung-Kai Lin; Hsing-Ju Wu; Nguyen Van Hieu; Pei-Yi Chu; Thi Vien Thao Do; Fiona Yan-Dong Yao; Thien Luan Phan; Congo Tak Shing Ching

doi:10.3390/bios12080561

A New Biorecognition-Element-Free IDμE Sensor for the Identification and Quantification of E. coli

Biosensors (Basel). 2022 Jul 25;12(8):561. doi: 10.3390/bios12080561.

Authors

Yung-Kai Lin^{1

2

3}, Hsing-Ju Wu^{4

5}, Nguyen Van Hieu⁶, Pei-Yi Chu^{7

8}, Thi Vien Thao Do^{3

6}, Fiona Yan-Dong Yao⁹, Thien Luan Phan^{3

6}, Congo Tak Shing Ching^{3

10}

Affiliations

¹ Institute of Food Safety and Risk Management, National Taiwan Ocean University, Keelung City 202, Taiwan.
² Department of Food Science, National Taiwan Ocean University, Keelung City 202, Taiwan.
³ Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung City 402, Taiwan.
⁴ Research Assistant Center, Show Chwan Memorial Hospital, Changhua City 500, Taiwan.
⁵ Department of Medical Research, Chang Bing Show Chwan Memorial Hospital, Lukang Township 505, Taiwan.
⁶ Department of Physics and Electronic Engineering, University of Science (Vietnam National University of Ho Chi Minh City), Ho Chi Minh City 700000, Vietnam.
⁷ Department of Pathology, Show Chwan Memorial Hospital, Changhua City 500, Taiwan.
⁸ School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan.
⁹ Division of Science, Engineering and Health Studies, The Hong Kong Polytechnic University-Hong Kong Community College, Hong Kong.
¹⁰ Department of Electrical Engineering, National Chi Nan University, Puli Township 545, Taiwan.

Abstract

The label-free biosensor has emerged as an effective tool for the purpose of early detection of causative pathogens such as Escherichia coli as a preventive measure. In this study, a biorecognition-element-free interdigitated microelectrode (IDμE) sensor is designed and developed with this in mind, with good reliability and affordability. Results show that the designed sensor can identify E. coli with good selectivity using an impedance and capacitance of 7.69 MHz. At its optimum impedance of 1.3 kHz, the IDμE sensor can reliably quantify E. coli in a range of measurement (10^3.2~10⁶ cfu/mL), linearity (R² = 0.97), sensitivity (18.15 kΩ/log (cfu/mL)), and limit of detection (10^3.2 cfu/mL). In summary, the IDμE sensor developed possesses high potential for industrial and clinical applications.

Keywords: Escherichia coli; biorecognition element free; capacitance; impedance; interdigitated microelectrodes.

MeSH terms

Biosensing Techniques* / methods
Electric Impedance
Escherichia coli O157*
Microelectrodes
Reproducibility of Results

Abstract

MeSH terms

Grants and funding