Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices

Juan Zhang; Wentao Ha; Yuhan Chang; Yutong Ge; Xiaowei Wei; Youhua Tan; Qiong Jia

doi:10.1016/j.xpro.2022.101577

Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices

STAR Protoc. 2022 Jul 19;3(3):101577. doi: 10.1016/j.xpro.2022.101577. eCollection 2022 Sep 16.

Authors

Juan Zhang¹, Wentao Ha², Yuhan Chang³, Yutong Ge³, Xiaowei Wei³, Youhua Tan⁴, Qiong Jia⁵

Affiliations

¹ Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China. Electronic address: lesleyzj@njmu.edu.cn.
² Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China. Electronic address: hawentao111@126.com.
³ Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
⁴ The Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen 518000, China; Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hong Kong 999077, China.
⁵ Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China. Electronic address: jiaqiong2020@njmu.edu.cn.

Abstract

Soft fibrin gels are used to select tumorigenic cells and regulate the stemness and metastasis of colorectal cancer cells via mechanotransduction. This protocol details steps to produce two-dimensional (2D) and three-dimensional (3D) extracellular matrices for substrate rigidity manipulation and tumorigenic cell selection. We also describe how it can be applied to tumor mechanotransductive research by colony growth monitoring and cell isolation. For complete details on the use and execution of this protocol, please refer to Chang et al. (2022).

Keywords: Biophysics; Biotechnology and bioengineering; Cancer; Cell Biology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Separation
Extracellular Matrix* / physiology
Fibrin
Gels
Mechanotransduction, Cellular*

Substances

Gels
Fibrin