Simultaneous determination of succinate-dehydrogenase-inhibitor fungicide traces in cereals by QuEChERS preparation and UPLC-MS/MS analysis

Haoran Zhao; Minmin Li; Xiaowei Liu; Jiajie Yang; Xueyao Li; Jieyin Chen; Xiaofeng Dai; Jesus Simal-Gandara; Zhiqiang Kong; Zhizhong Li

doi:10.1016/j.foodchem.2022.133708

Simultaneous determination of succinate-dehydrogenase-inhibitor fungicide traces in cereals by QuEChERS preparation and UPLC-MS/MS analysis

Food Chem. 2022 Dec 1:396:133708. doi: 10.1016/j.foodchem.2022.133708. Epub 2022 Jul 16.

Authors

Haoran Zhao¹, Minmin Li², Xiaowei Liu³, Jiajie Yang⁴, Xueyao Li⁵, Jieyin Chen⁶, Xiaofeng Dai⁶, Jesus Simal-Gandara⁷, Zhiqiang Kong⁸, Zhizhong Li⁹

Affiliations

¹ School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
² Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
³ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
⁴ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; College Life Science & Technology, Xinjiang University, 830046 Shengli Road, Urumqi, China.
⁵ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; College of Food Science and Technology, Hebei Agricultural University, Baoding 071000, Hebei, China.
⁶ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji 831100, China.
⁷ Universidade de Vigo, Nutrition and Bromatology Group, Analytical Chemistry and Food Science Department, Faculty of Science, E32004 Ourense, Spain. Electronic address: jsimal@uvigo.es.
⁸ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji 831100, China. Electronic address: kongzhiqiang@caas.cn.
⁹ School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China. Electronic address: zzli2004@lut.edu.cn.

PMID: 35878445
DOI: 10.1016/j.foodchem.2022.133708

Abstract

A method for the simultaneous determination of 19 succinate dehydrogenase inhibitor (SDHI) fungicide residues in 8 kinds of cereals was established by combining UHPLC-MS/MS with the improved QuEChERS method. MgSO₄ and octadecylsilane (C₁₈) were used as the dispersive-solid phase extraction sorbent. The proposed method had good linearity in the range of 10-100 µg/L with correlation coefficients (R² > 0.99). The limit of quantification of 19 fungicides was 10 µg/L, which is the minimum addition level of the method. The fortified recoveries of 19 SDHI fungicides at three levels were ranged from 79.57 % to 126.25 %. The developed method was utilized for the analysis of 45 real cereal samples, only 5 samples were detected with SDHI fungicides. The contents of the fungicides detected in the real samples are far lower than the MRL. The results indicated that the proposed method is reliable for detecting SDHI fungicides in cereals.

Keywords: Cereals; Modified QuEChERS; Multiresidues; Succinate dehydrogenase inhibitor fungicides; UHPLC-MS/MS.

MeSH terms

Chromatography, High Pressure Liquid
Chromatography, Liquid / methods
Edible Grain / chemistry
Fungicides, Industrial* / analysis
Succinate Dehydrogenase / analysis
Succinic Acid / analysis
Tandem Mass Spectrometry* / methods

Substances

Fungicides, Industrial
Succinic Acid
Succinate Dehydrogenase