A new furostane saponin, ramosaponin (1), and four known furostane saponins, protodioscin (2), dehydrotomatoside (3), (25 R)-26-O-(β-D-glucopyranosyl)-furost-5-ene-3β,22α,26-triol 3-O-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside (4), and anguivioside A (5) were isolated from the methanol extract of Allium ramosum seeds. Their structures were identified based on spectroscopic evidence and comparison with those reported in the literature. All compounds were evaluated for reduction of lipid accumulation in HepG2 cell line. As a result, compound 1 showed significant lipid accumulation inhibitory activity with an IC50 value of 64.32 ± 3.87 µM.
Keywords: Allium ramosum; Amaryllidaceae; furostane saponin; lipid accumulation; ramosaponin.