SoyMAGIC: An Unprecedented Platform for Genetic Studies and Breeding Activities in Soybean

Seyed Mohammad Hashemi; Gregory Perry; Istvan Rajcan; Milad Eskandari

doi:10.3389/fpls.2022.945471

SoyMAGIC: An Unprecedented Platform for Genetic Studies and Breeding Activities in Soybean

Front Plant Sci. 2022 Jul 7:13:945471. doi: 10.3389/fpls.2022.945471. eCollection 2022.

Authors

Seyed Mohammad Hashemi¹, Gregory Perry¹, Istvan Rajcan¹, Milad Eskandari¹

Affiliation

¹ Department of Plant Agriculture, Ontario Agriculture College, University of Guelph, Guelph, ON, Canada.

Abstract

Multi-Parent Advanced Generation Inter-Cross (MAGIC) populations are emerging genetic platforms for high-resolution and fine mapping of quantitative traits, such as agronomic and seed composition traits in soybean (Glycine max L.). We have established an eight-parent MAGIC population, comprising 721 recombinant inbred lines (RILs), through conical inter-mating of eight soybean lines. The parental lines were genetically diverse elite cultivars carrying different agronomic and seed composition characteristics, including amino acids and fatty acids, as well as oil and protein concentrations. This study aimed to introduce soybean MAGIC (SoyMAGIC) population as an unprecedented platform for genotypic and phenotypic investigation of agronomic and seed quality traits in soybean. The RILs were evaluated for important seed composition traits using replicated field trials during 2020 and 2021. To measure the seed composition traits, near-infrared reflectance (NIR) was employed. The RILs were genotyped using genotyping-by-sequencing (GBS) method to decipher the genome and discover single-nucleotide polymorphic (SNP) markers among the RILs. A high-density linkage map was constructed through inclusive composite interval mapping (ICIM). The linkage map was 3,770.75 cM in length and contained 12,007 SNP markers. Chromosomes 11 and 18 were recorded as the shortest and longest linkage groups with 71.01 and 341.15 cM in length, respectively. Observed transgressive segregation of the selected traits and higher recombination frequency across the genome confirmed the capability of MAGIC population in reshuffling the diversity in the soybean genome among the RILs. The assessment of haplotype blocks indicated an uneven distribution of the parents' genomes in RILs, suggesting cryptic influence against or in favor of certain parental genomes. The SoyMAGIC population is a recombined genetic material that will accelerate further genomic studies and the development of soybean cultivars with improved seed quality traits through the development and implementation of reliable molecular-based toolkits.

Keywords: Seed composition/quality; genetic linkage map; genotyping by sequencing; multi-parent advanced generation inter-crosses; soybean (Glycine max L.).