Global Integrated Genomic and Transcriptomic Analyses of MYB Transcription Factor Superfamily in C3 Model Plant Oryza sativa (L.) Unravel Potential Candidates Involved in Abiotic Stress Signaling

Pandiyan Muthuramalingam; Rajendran Jeyasri; Anthonymuthu Selvaraj; Hyunsuk Shin; Jen-Tsung Chen; Lakkakula Satish; Qiang-Sheng Wu; Manikandan Ramesh

doi:10.3389/fgene.2022.946834

Global Integrated Genomic and Transcriptomic Analyses of MYB Transcription Factor Superfamily in C3 Model Plant Oryza sativa (L.) Unravel Potential Candidates Involved in Abiotic Stress Signaling

Front Genet. 2022 Jul 8:13:946834. doi: 10.3389/fgene.2022.946834. eCollection 2022.

Authors

Pandiyan Muthuramalingam^{1

2

3}, Rajendran Jeyasri¹, Anthonymuthu Selvaraj^{1

4}, Hyunsuk Shin^{2

3}, Jen-Tsung Chen⁵, Lakkakula Satish⁶, Qiang-Sheng Wu⁷, Manikandan Ramesh¹

Affiliations

¹ Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, India.
² Department of Horticultural Science, Gyeongsang National University, Jinju, South Korea.
³ Department of GreenBio Science, Gyeongsang National University, Jinju, South Korea.
⁴ Department of Physiology and Biophysics, University of California, Irvine, Irvine, CA, United States.
⁵ Department of Life Sciences, National University of Kaohsiung, Kaohsiung, Taiwan.
⁶ Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer Sheva, Israel.
⁷ College of Horticulture and Gardening, Yangtze University, Jingzhou, China.

Abstract

Plant transcription factors (TFs) are significant players in transcriptional regulations, signal transduction, and constitute an integral part of signaling networks. MYB TFs are major TF superfamilies that play pivotal roles in regulation of transcriptional reprogramming, physiological processes, and abiotic stress (AbS) responses. To explore the understanding of MYB TFs, genome and transcriptome-wide identification was performed in the C3 model plant, Oryza sativa (OsMYB). This study retrieved 114 OsMYB TFs that were computationally analyzed for their expression profiling, gene organization, cis-acting elements, and physicochemical properties. Based on the microarray datasets, six OsMYB genes which were sorted out and identified by a differential expression pattern were noted in various tissues. Systematic expression profiling of OsMYB TFs showed their meta-differential expression of different AbS treatments, spatio-temporal gene expression of various tissues and their growth in the field, and gene expression profiling in responses to phytohormones. In addition, the circular ideogram of OsMYB genes in related C4 grass plants conferred the gene synteny. Protein-protein interactions of these genes revealed the molecular crosstalk of OsMYB TFs. Transcriptional analysis (qPCR) of six OsMYB players in response to drought and salinity stress suggested the involvement in individual and combined AbS responses. To decipher how these OsMYB play functional roles in AbS dynamics, further research is a prerequisite.

Keywords: MYB; Oryza sativa; abiotic stress; comparative mapping; phytohormone; qPCR; spatio-temporal; transcription factors.