Genetic variation and evolution of attenuated African swine fever virus strain isolated in the field: A review

Wang Zhenzhong; Qi Chuanxiang; Ge Shengqiang; Li Jinming; Hu Yongxin; Zhang Xiaoyue; Lv Yan; Han Naijun; Wu Xiaodong; Wang Zhiliang; Qian Yingjuan

doi:10.1016/j.virusres.2022.198874

Genetic variation and evolution of attenuated African swine fever virus strain isolated in the field: A review

Virus Res. 2022 Oct 2:319:198874. doi: 10.1016/j.virusres.2022.198874. Epub 2022 Jul 21.

Authors

Wang Zhenzhong¹, Qi Chuanxiang², Ge Shengqiang³, Li Jinming⁴, Hu Yongxin⁵, Zhang Xiaoyue⁶, Lv Yan⁷, Han Naijun⁸, Wu Xiaodong⁹, Wang Zhiliang¹⁰, Qian Yingjuan¹¹

Affiliations

¹ MOE Joint International Research Laboratory for Animal Health and Food Safety/Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address: sanzudingli@126.com.
² MOE Joint International Research Laboratory for Animal Health and Food Safety/Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address: chuanxiangqi@foxmail.com.
³ China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, China. Electronic address: geshengqiang@cahec.cn.
⁴ China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, China.
⁵ China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, China. Electronic address: huyongxin@cahec.cn.
⁶ Shandong Agricultural University, Tai'an, Shandong 271001, China. Electronic address: 575254748@qq.com.
⁷ China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, China. Electronic address: lvyan@cahec.cn.
⁸ China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, China. Electronic address: sam920@163.com.
⁹ China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, China. Electronic address: wuxiaodong@cahec.cn.
¹⁰ China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, China. Electronic address: wangzhiliang@cahec.cn.
¹¹ MOE Joint International Research Laboratory for Animal Health and Food Safety/Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address: yqian@njau.edu.cn.

PMID: 35872281
DOI: 10.1016/j.virusres.2022.198874

Abstract

It has been reported that there were several "mutant isolated in the field " of African swine fever virus (ASFV) since ASFV was reported, which may be the result of the continuous adaptation and evolution of ASFV. The emergence of ASFV field mutants may lead to chronic or asymptomatic "atypical clinical symptoms" in pigs and hinder the development of porcine industry. Here we analyzed the published ASFV "field attenuated strain" gene sequences and reviewed the genetic differences between field attenuated and virulent ASFV strains, hoping for providing a reference for the scientific prevention and control of ASF and the development of new vaccines. In this study we found the deletion of EP153R and EP402R occurred in 4 field attenuated strains, and all the differential genes of field attenuated strains mainly range in regions with low GC content. The evolution of MGF110 family genes was identified by analysis of two field attenuated ASFV strains from Portugal. We also found that some tandem repeat sequence plays an important role in the evolution of strains of NH/P68 and OURT 88/3 but not in strains Estonia 2014, HuB20 and Pig/Heilongjiang/HRB1/2020.

Keywords: ASF; Field variant strains; Sequence analysis.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

African Swine Fever Virus*
African Swine Fever*
Animals
Genetic Variation
Swine
Viral Proteins / genetics
Viral Vaccines*

Substances

Viral Proteins
Viral Vaccines