Analysis of metabolites in green tea during the roasting process using non-targeted metabolomics

J Sci Food Agric. 2023 Jan 15;103(1):213-220. doi: 10.1002/jsfa.12133. Epub 2022 Aug 11.

Abstract

Background: Roasting plays an important role in the formation of flavor of roasted green tea; however, the changes in chemicals during this process have not been systematically studied until now. To reveal the dynamic changes in chemicals in green tea during roasting, non-targeted metabolomics, coupled with chemometrics, was employed.

Results: A total of 101 non-volatile metabolites were identified in tea samples, and 29 metabolites were identified as characteristic metabolites of roasting. A significant increase in catechins and their derivatives, organic acids, and flavonoid glycosides was observed, while the content of some amino acids and their derivatives decreased over 50% during roasting. The content of theanine glucoside increased dramatically (by 21.23-fold at the roasting stage), and Maillard-derived compounds also increased to varying degrees.

Conclusion: Glycosylation, oxidative polymerization, and pyrolysis were important reactions responsible for the formation and transformation of flavor compounds in roasted green tea during roasting. © 2022 Society of Chemical Industry.

Keywords: flavonoids glycosides; non-targeted metabolomics; roasted green tea; roasting process; thermal degradation.

MeSH terms

  • Catechin*
  • Hot Temperature
  • Metabolomics
  • Tea* / chemistry

Substances

  • Tea
  • Catechin