Immunophenotypic characterization of B1a lymphocytes in Cuban older adults

Vianed Marsán Suárez; Imilla Casado Hernández; Elizabeth Hernández Ramos; Gabriela Díaz Domínguez; Yenisey Triana Marrero; Yaneisy Duarte Pérez; Arturo Chang Monteagudo; Ana María Simón Pita; Yaquima de Los Milagros Hernández Rego; Mary Carmen Reyes Zamora; Luis Felipe Heredia Guerra; Consuelo Milagros Macías Abraham

doi:10.1016/j.exger.2022.111900

Immunophenotypic characterization of B1a lymphocytes in Cuban older adults

Exp Gerontol. 2022 Oct 1:167:111900. doi: 10.1016/j.exger.2022.111900. Epub 2022 Jul 20.

Affiliations

¹ Department of Immunology, Institute of Hematology and Immunology "José Manuel Ballester Santovenia", Calle 19 e/ 8 y 10, PO Box 8070, CP 10800 Vedado, Havana, Cuba. Electronic address: vianedmarsansuarez68@gmail.com.
² Department of Immunology, Institute of Hematology and Immunology "José Manuel Ballester Santovenia", Calle 19 e/ 8 y 10, PO Box 8070, CP 10800 Vedado, Havana, Cuba.
³ Department of Histocompatibility, Institute of Hematology and Immunology "José Manuel Ballester Santovenia", Calle 19 between 8 and 10, PO Box 8070, CP 10800 Vedado, Havana, Cuba.
⁴ Department of Morphology and Pathology, Institute of Hematology and Immunology "José Manuel Ballester Santovenia", Calle 19 between 8 and 10, PO Box 8070, CP 10800 Vedado, Havana, Cuba.
⁵ National Center for Biopreparations, Bejucal, Mayabeque, Cuba.
⁶ Research Center for the Elderly (CITED), Vedado, Havana, Cuba.
⁷ Institute of Hematology and Immunology Institute "José Manuel Ballester Santovenia", Calle 19 between 8 and 10, PO Box 8070, CP 10800 Vedado, Havana, Cuba; Hematology and Immunology "José Manuel Ballester Santovenia", Calle 19 e/ 8 y 10, PO Box 8070, CP 10800 Vedado, Havana, Cuba.

PMID: 35868536
DOI: 10.1016/j.exger.2022.111900

Abstract

Introduction: B1a lymphocytes are characterized by having a high capacity for self-renewal and production of natural antibodies, in a T-independent manner. There are differences in both the number and composition of mature B lymphocytes throughout life, due to the phenomenon of immunosenescence.

Objective: To characterize the immunophenotype of B1a lymphocytes in older Cuban adults with a simplified CD19CD20CD5 panel.

Methods: A cross-sectional study was conducted in 30 institutionalized Cuban older adults. Characterization of CD19 + CD5+, CD20 + CD5+, and CD19 + CD20+ B lymphocytes in peripheral blood was performed by flow cytometry. For the reading and analysis of the biological samples, a flow cytometer, Beckman Coulter, Gallios, was used. A Microsoft Excel database was created with the information obtained. The sample was divided by sex and age. The data were processed using the statistical program GraphPadPrism version 6.00 using the percentage values and the median for the sample description.

Results: The elderly aged 80 years and over showed a decrease in the absolute count of CD19 + CD20+ B lymphocytes, as well as in the percentage and absolute count of CD19 + CD5+ B lymphocytes. On the other hand, they presented higher absolute counts of CD20 + CD5+ lymphocytes in relation to those of the <80-year-old group. Women showed higher absolute counts of CD19 + CD5+, CD20 + CD5+, and CD19 + CD20+ B lymphocyte populations.

Conclusions: The immunophenotypic characterization of B1a lymphocytes in older Cuban adults is similar to that reported by other researchers. Both age and sex influence the absolute count of these cells, being higher in women under 80 years of age.

Keywords: B1a lymphocytes; Flow cytometry; Immunosenescence; Older adults.

MeSH terms

Aged
Aged, 80 and over
B-Lymphocytes*
Cross-Sectional Studies
Female
Flow Cytometry
Humans
Lymphocytes*