RNA Sequencing Data Analysis on the Maser Platform and the Tag-Count Comparison Graphical User Interface

Kenta Ujifuku; Yoichi Morofuji; Hiroshi Masumoto

doi:10.1007/978-1-0716-2513-2_13

RNA Sequencing Data Analysis on the Maser Platform and the Tag-Count Comparison Graphical User Interface

Methods Mol Biol. 2022:2535:157-170. doi: 10.1007/978-1-0716-2513-2_13.

Authors

Kenta Ujifuku¹, Yoichi Morofuji², Hiroshi Masumoto³

Affiliations

¹ Department of Neurosurgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. kujifuku-nag@umin.ac.jp.
² Department of Neurosurgery, Nagasaki University Hospital, Nagasaki, Japan.
³ Biomedical research support center, Nagasaki University School of Medicine, Nagasaki, Japan.

PMID: 35867230
DOI: 10.1007/978-1-0716-2513-2_13

Abstract

The RNA sequencing (RNA-seq) process that allows for comprehensive transcriptome analysis has become increasingly simple. Analysis and interpretation of RNA-seq output data are indispensable for research, but bioinformatics experts are not always available to assist. Currently, however, even a wet-lab specialist can perform the pipeline analysis of RNA-seq described in this chapter using the Maser platform and the Tag-Count Comparison Graphical User Interface (TCC-GUI). These are free of charge for scientific use.

Keywords: Maser; RNA-seq; Spreadsheet software; TCC-GUI.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Data Analysis*
Gene Expression Profiling
High-Throughput Nucleotide Sequencing
Lasers
Sequence Analysis, RNA
Software*
User-Computer Interface