Optogenetic Control of Human Stem Cell-Derived Neurons

Rouhollah Habibey; Johannes Striebel; Kritika Sharma; Volker Busskamp

doi:10.1007/978-1-0716-2329-9_17

Optogenetic Control of Human Stem Cell-Derived Neurons

Methods Mol Biol. 2022:2501:339-360. doi: 10.1007/978-1-0716-2329-9_17.

Authors

Rouhollah Habibey¹, Johannes Striebel¹, Kritika Sharma¹, Volker Busskamp²

Affiliations

¹ Department of Ophthalmology, Universitäts-Augenklinik Bonn, University of Bonn, Bonn, Germany.
² Department of Ophthalmology, Universitäts-Augenklinik Bonn, University of Bonn, Bonn, Germany. Volker.busskamp@uni-bonn.de.

PMID: 35857237
DOI: 10.1007/978-1-0716-2329-9_17

Abstract

Spontaneous and optogenetically evoked activities of human induced pluripotent stem cell (hiPSC)-derived neurons can be assessed by patch clamp and multi-electrode array (MEA) electrophysiology. Optogenetic activation of these human neurons facilitates the characterization of their functional properties at the single neuron and circuit level. Here we showcase the preparation of hiPSC-derived neurons expressing optogenetic actuators, in vitro optogenetic stimulation and simultaneous functional recordings using patch clamp and MEA electrophysiology.

Keywords: Banker culture; Induced pluripotent stem cells (iPSCs); Long-term iPSC-derived neuronal culture; Multi-electrode array (MEA) electrophysiology; Neuron-astrocyte co-culture; Optogenetic stimulation; Patch clamp.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Action Potentials / physiology
Cell Differentiation / genetics
Cells, Cultured
Humans
Induced Pluripotent Stem Cells*
Neurons
Optogenetics*