Green synthesis of silver nanoparticles using Cinnamomum tamala (Tejpata) leaf and their potential application to control multidrug resistant Pseudomonas aeruginosa isolated from hospital drainage water

Md Abdullah Al Mashud; Md Moinuzzaman; Md Shamim Hossain; Sabbir Ahmed; Galib Ahsan; Abu Reza; Robayet Bin Anwar Ratul; Md Helal Uddin; Md Abdul Momin; Mohammad Abu Hena Mostofa Jamal

doi:10.1016/j.heliyon.2022.e09920

Green synthesis of silver nanoparticles using Cinnamomum tamala (Tejpata) leaf and their potential application to control multidrug resistant Pseudomonas aeruginosa isolated from hospital drainage water

Heliyon. 2022 Jul 9;8(7):e09920. doi: 10.1016/j.heliyon.2022.e09920. eCollection 2022 Jul.

Authors

Md Abdullah Al Mashud^{1

2}, Md Moinuzzaman^{1

2}, Md Shamim Hossain^{3

4}, Sabbir Ahmed^{3

4}, Galib Ahsan³, Abu Reza^{3

4}, Robayet Bin Anwar Ratul^{3

4}, Md Helal Uddin⁵, Md Abdul Momin¹, Mohammad Abu Hena Mostofa Jamal^{3

4}

Affiliations

¹ Department of Electrical and Electronic Engineering, Faculty of Engineering and Technology, Islamic University, Kushtia 7003, Bangladesh.
² Biophysics and Biomedicine Research Lab, Faculty of Engineering and Technology, Islamic University, Kushtia 7003, Bangladesh.
³ Department Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh.
⁴ Laboratory of Environmental and Clinical Microbiology, Department Biotechnology and Genetic Engineering, Islamic University, Kushtia 7003, Bangladesh.
⁵ Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering and Technology, Islamic University, Kushtia 7003, Bangladesh.

Abstract

Green Synthesis of Metal Nanoparticles is becoming a more common method for producing nanoparticles with a diameter of 1-100 nm that may be employed in a variety of medical applications. The antibacterial efficacy of silver nanoparticles (AgNPs) derived from Cinnamomum tamala (Tejpata) leaf extract against antibiotic-resistant Pseudomonas aeruginosa is investigated in this study. Green AgNP synthesis is safe, cost-effective, and ecologically friendly. The biosynthesized AgNPs were studied using UV-Visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), Dynamic Light Scattering (DLS), X-ray Diffraction (XRD), and Transmission Electron Microscopy (TEM). The AgNPs were virtually spherical, with an average size of 25-30 nm, according to TEM observations. Biochemical and molecular identification were used to isolate multidrug-resistant P. aeruginosa from the hospital's drainage water. The antibacterial potential of AgNPs against P. aeruginosa is determined using the agar diffusion method. Silver nanoparticles produced from Cinnamomum tamala (Tejpata) leaf extract were shown to be effective in inhibiting four strains of P. aeruginosa. According to the agar disc diffusion method, AgNPs had the largest inhibition zone of 17.67 ± 0.577 mm, while aqueous extract had 5.67 ± 0.5777 mm, indicating that AgNPs had antibacterial activity. This study on AgNPs might assist with managing multidrug resistant pathogenic bacteria and be a possible source of medicinal application due to its potential antibacterial effect.

Keywords: Antibacterial activity; Antibiotic resistance; Green synthesis; Leaf extract; Silver nanoparticles.