Toehold-controlled ligation and transcription for accurate COVID-19 genotyping

Yanmin Gao; Taoxue Wang; Jiaojiao Li; Yanan Wei; Hao Qi

doi:10.1016/j.ab.2022.114803

Toehold-controlled ligation and transcription for accurate COVID-19 genotyping

Anal Biochem. 2022 Oct 1:654:114803. doi: 10.1016/j.ab.2022.114803. Epub 2022 Jul 15.

Authors

Yanmin Gao¹, Taoxue Wang¹, Jiaojiao Li¹, Yanan Wei¹, Hao Qi²

Affiliations

¹ School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China; Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300350, China; Zhejiang Shaoxing Research Institute of Tianjin University, Zhejiang, 312369, China.
² School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China; Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300350, China; Zhejiang Shaoxing Research Institute of Tianjin University, Zhejiang, 312369, China. Electronic address: haoq@tju.edu.cn.

Abstract

The global pandemic of coronavirus disease 2019 (COVID-19) has significant impact on the entire human society. However, in the face of continually emerging more contagious SARS-CoV-2 variant, the risk to bog down into more severe crisis is around us anytime. Here, we introduce an isothermal, ultrasensitive method for identifying important SNV mutations of SARS-CoV-2. It is based on combined specificity of toehold-assisted linear probe ligation and in vitro transcription signal enlargement, TLT. A ready-to-use panel of TLT assay is developed including detection of 80 crucial SARS-CoV-2 SNVs, by which people could response to the next coming contagious virus variant more rapidly. These advanced point-of-care features make TLT one good approach for large scale population testing of special SARS-CoV-2 variants of interesting.

Keywords: COVID-19; Ligase chain reaction; SARS-CoV-2; Toehold-mediated strand displacement reaction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

COVID-19 Testing
COVID-19* / diagnosis
Clinical Laboratory Techniques / methods
Genotype
Humans
Nucleic Acid Amplification Techniques / methods
SARS-CoV-2* / genetics
Sensitivity and Specificity

Supplementary concepts

SARS-CoV-2 variants