A Rapid User-Friendly Lab-on-a-Chip Microarray Platform for Detection of SARS-CoV-2 Variants

Methods Mol Biol. 2022:2511:117-131. doi: 10.1007/978-1-0716-2395-4_9.

Abstract

Since the original SARS-CoV-2 virus emerged from Wuhan, China, in late December 2019, a number of variants have arisen with enhanced infectivity, and some may even be capable of escaping the existing vaccines. Here we describe a rapid automated nucleic acid microarray hybridization and readout in less than 15 min using the Fraunhofer lab-on-a-chip platform for identification of bacterial species and antibiotic resistance. This platform allows a fast adaptation of new biomarkers enabling identification of different genes and gene mutations, such as those seen in the case the SARS-CoV-2 variants.

Keywords: Biomarker; COVID-19; LOC; Lab-on-a-chip; Microarray; SARS-CoV-2; Variant.

MeSH terms

  • COVID-19* / diagnosis
  • China
  • Humans
  • Lab-On-A-Chip Devices
  • SARS-CoV-2* / genetics

Supplementary concepts

  • SARS-CoV-2 variants