In Vivo Bioluminescent Imaging of Bone Marrow-Derived Mesenchymal Stem Cells in Mice

Prakash Gangadaran; Ji Min Oh; Ramya Lakshmi Rajendran; Byeong-Cheol Ahn

doi:10.1007/978-1-0716-2473-9_21

In Vivo Bioluminescent Imaging of Bone Marrow-Derived Mesenchymal Stem Cells in Mice

Methods Mol Biol. 2022:2525:281-287. doi: 10.1007/978-1-0716-2473-9_21.

Authors

Prakash Gangadaran^#^{1

2}, Ji Min Oh^#², Ramya Lakshmi Rajendran², Byeong-Cheol Ahn^{3

4

5}

Affiliations

¹ BK21 FOUR KNU Convergence Educational Program of Biomedical Sciences for Creative Future Talents, Department of Biomedical Science, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
² Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
³ BK21 FOUR KNU Convergence Educational Program of Biomedical Sciences for Creative Future Talents, Department of Biomedical Science, School of Medicine, Kyungpook National University, Daegu, Republic of Korea. abc2000@knu.ac.kr.
⁴ Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea. abc2000@knu.ac.kr.
⁵ Department of Nuclear Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea. abc2000@knu.ac.kr.

^# Contributed equally.

PMID: 35836076
DOI: 10.1007/978-1-0716-2473-9_21

Abstract

Mesenchymal stem cells (MSCs) are multipotent adult stem cells present in multiple tissues, such as bone marrow, adipose tissue, umbilical cord, and amniotic fluid. MSCs can differentiate into multilineage cells under defined conditions in vitro and in vivo. MSCs have been shown to have therapeutic effects on various types of diseases. Noninvasive in vivo monitoring of MSCs is considered one of the important techniques for developing cell therapy. In this protocol, we introduce strategized MSCs derived from bone marrow (BM-MSCs) of knock-in mouse model expressing mCherry-Renilla luciferase (mCherry-RLuc) for noninvasive bioluminescence imaging (BLI) of injected BM-MSCs in vivo.

Keywords: Bioluminescence (BL); Bone marrow stem cells; In vivo imaging; Renilla luciferase (RLuc).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Marrow
Bone Marrow Cells*
Cell Differentiation
Cell Proliferation
Mesenchymal Stem Cells*
Mice