Metagenomics reveals the metabolism of polyphosphate-accumulating organisms in biofilm sequencing batch reactor: A new model

Min Ni; Yang Pan; Dapeng Li; Yong Huang; Zhiqiang Chen; Lu Li; Zuowei Song; Yimeng Zhao

doi:10.1016/j.biortech.2022.127603

Metagenomics reveals the metabolism of polyphosphate-accumulating organisms in biofilm sequencing batch reactor: A new model

Bioresour Technol. 2022 Sep:360:127603. doi: 10.1016/j.biortech.2022.127603. Epub 2022 Jul 12.

Authors

Min Ni¹, Yang Pan², Dapeng Li³, Yong Huang³, Zhiqiang Chen⁴, Lu Li³, Zuowei Song⁵, Yimeng Zhao⁶

Affiliations

¹ School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China; Jiangsu Key Laboratory of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
² School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Suzhou University of Science and Technology, Suzhou 215009, China. Electronic address: panyang@mail.usts.edu.cn.
³ School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Suzhou University of Science and Technology, Suzhou 215009, China.
⁴ Harbin Institute of Technology, Harbin 150006, China.
⁵ Novoprotein, Suzhou 215200, China.
⁶ School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.

PMID: 35835418
DOI: 10.1016/j.biortech.2022.127603

Abstract

This study assessed the impact of the operating conditions of the biofilm sequencing batch reactor (BSBR) on the community structure and the growth/metabolic pathways of its polyphosphate-accumulating organisms (PAOs). There are significant difference with reference to the enhanced biological phosphorus removal (EBPR) process. The leading PAOs in BSBR generally are capable of high affinity acetate metabolism, gluconeogenesis, and low affinity phosphate transport, and have various carbon source supplementation pathways to ensure the efficient circulation of energy and reducing power. A new model of the metabolic mechanism of PAOs in the BSBR was formulated, which features low glycogen metabolism with simultaneous gluconeogenesis and glycogenolysis and differs significantly from the classic mechanism based on Candidatus_Accumulibacter and Tetrasphaera. The findings will assist the efficient recovery of low concentration phosphate in municipal wastewater.

Keywords: Biofilm sequencing batch reactor; Gluconeogenesis; Metabolic pathways; Polyphosphate-accumulating organisms; Process performance.

Publication types

Review

MeSH terms

Biofilms
Bioreactors*
Metagenomics
Phosphorus / metabolism
Polyphosphates* / metabolism

Substances

Polyphosphates
Phosphorus