Quantitative and amplification-free detection of SOCS-1 CpG methylation percentage analyses in gastric cancer by fiber optic nanoplasmonic biosensor

Lakshmi Sujatha Guthula; Kun-Tu Yeh; Wen-Long Huang; Chun-Hsien Chen; Yen-Ling Chen; Chun-Jen Huang; Lai-Kwan Chau; Michael W Y Chan; Shu-Hui Lin

doi:10.1016/j.bios.2022.114540

Quantitative and amplification-free detection of SOCS-1 CpG methylation percentage analyses in gastric cancer by fiber optic nanoplasmonic biosensor

Biosens Bioelectron. 2022 Oct 15:214:114540. doi: 10.1016/j.bios.2022.114540. Epub 2022 Jul 6.

Authors

Lakshmi Sujatha Guthula¹, Kun-Tu Yeh², Wen-Long Huang³, Chun-Hsien Chen⁴, Yen-Ling Chen⁵, Chun-Jen Huang⁶, Lai-Kwan Chau⁷, Michael W Y Chan⁸, Shu-Hui Lin⁹

Affiliations

¹ Department of Chemistry and Biochemistry, National Chung Cheng University, Chiayi, Taiwan.
² Department of Surgical Pathology, Changhua Christian Hospital, Changhua, Taiwan; College of Medicine, National Chung Hsiung University, Taichung, Taiwan.
³ Department of Biomedical Sciences, National Chung Cheng University, Chiayi, Taiwan.
⁴ School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.
⁵ Department of Chemistry and Biochemistry, National Chung Cheng University, Chiayi, Taiwan; School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan; Center for Nano Bio-Detection, National Chung Cheng University, Chiayi, Taiwan.
⁶ Department of Chemical and Materials Engineering, NCU-Covestro Research Center, National Central University, Taoyuan, Taiwan; R&D Center for Membrane Technology, Chung Yuan Christian University, Taoyuan, Taiwan.
⁷ Department of Chemistry and Biochemistry, National Chung Cheng University, Chiayi, Taiwan; Center for Nano Bio-Detection, National Chung Cheng University, Chiayi, Taiwan; Center for Innovative Research on Aging Society (CIRAS), National Chung Cheng University, Chiayi, Taiwan. Electronic address: chelkc@ccu.edu.tw.
⁸ Department of Biomedical Sciences, National Chung Cheng University, Chiayi, Taiwan; Center for Nano Bio-Detection, National Chung Cheng University, Chiayi, Taiwan; Center for Innovative Research on Aging Society (CIRAS), National Chung Cheng University, Chiayi, Taiwan; Epigenomics and Human Disease Research Center, National Chung Cheng University, Chiayi, Taiwan. Electronic address: biowyc@ccu.edu.tw.
⁹ Department of Surgical Pathology, Changhua Christian Hospital, Changhua, Taiwan; Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, Taiwan. Electronic address: 74630@cch.org.tw.

PMID: 35834975
DOI: 10.1016/j.bios.2022.114540

Abstract

A new innovative approach is essential for early and effective diagnosis of gastric cancer, using promoter hypermethylation of the tumor suppressor, SOCS-1, that is frequently inactivated in human cancers. We have developed an amplification-free fiber optic nanoplasmonic biosensor for detecting DNA methylation of the SOCS-1 human genome. The method is based on the fiber optic nanogold-linked sorbent assay of PCR-free DNA from human gastric tumor tissue and cell lines. We designed a specific DNA probe fabricated on the fiber core surface while the other probe is bioconjugated with gold nanoparticles in free form to allow percentage determination and differentiating the methylated and unmethylated cell lines, further demonstrating the SOCS-1 methylation occurs in cancer patients but not in normal cell lines. The observed detection limit is 0.81 fM for methylated DNA, and the detection time is within 15 min. In addition, our data were significantly correlated to the data obtained from PCR-based pyrosequencing, and yet with superior accuracy. Hence our results provide new insight to the quantitative evaluation of methylation status of the human genome and can act as an alternative to PCR with a great potential.

Keywords: DNA methylation; Fiber optic biosensor; Gastric cancer; Gold nanoparticle; Localized surface plasmon resonance; SOCS-1.

MeSH terms

Biosensing Techniques*
CpG Islands
DNA / metabolism
DNA Methylation
Gold
Humans
Metal Nanoparticles*
Stomach Neoplasms* / diagnosis
Stomach Neoplasms* / genetics
Suppressor of Cytokine Signaling 1 Protein / genetics
Suppressor of Cytokine Signaling 1 Protein / metabolism
Suppressor of Cytokine Signaling Proteins / genetics
Suppressor of Cytokine Signaling Proteins / metabolism

Substances

Suppressor of Cytokine Signaling 1 Protein
Suppressor of Cytokine Signaling Proteins
Gold
DNA