Association of rs1544410 and rs7975232 Polymorphisms and Serum Vitamin D Levels with Psoriasis Susceptibility and Severity: A Case-Control Study in Egyptian Patients

Amal Ahmed Mohamed; Eman Elhussain; Naglaa Fawzy; Yasser Sakr; Marwa Salah El-Dien; Abbas Mohammed Abbas; Maha S Hussein; Nourelhuda Nassar; Omnia Ezzat; Reham Yousry El-Amir; Sarah Ibrahim; Nermeen Ibrahim Bedair

doi:10.2147/CCID.S364267

Association of rs1544410 and rs7975232 Polymorphisms and Serum Vitamin D Levels with Psoriasis Susceptibility and Severity: A Case-Control Study in Egyptian Patients

Clin Cosmet Investig Dermatol. 2022 Jul 7:15:1271-1281. doi: 10.2147/CCID.S364267. eCollection 2022.

Authors

Affiliations

¹ Department of Biochemistry and Molecular Biology, National Hepatology and Tropical Medicine Research institute, Cairo, Egypt.
² Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Giza, Egypt.
³ Department of Clinical Pathology, National Institute of Diabetics and Endocrinology, Cairo, Egypt.
⁴ Department of Community, Environmental and Occupational Medicine, Faculty of Medicine, Benha university, Banha, Egypt.
⁵ Department of Biochemistry, Faculty of Medicine, Cairo University, Giza, Egypt.
⁶ Department of Dermatology and Andrology, Medical Research and Clinical Studies Institute, National Research Center, Cairo, Egypt.
⁷ Department of Clinical Pathology, Elsahel Teaching Hospital, Cairo, Egypt.
⁸ Department of Biochemistry, Faculty of Pharmacy, Egyptian Russian University, Cairo, Egypt.
⁹ Department of Public Health, Faculty of Medicine, Cairo University, Giza, Egypt.
¹⁰ Department of Dermatology, Faculty of Medicine, Cairo university, Cairo, Egypt.
¹¹ Department of Dermatology, Andrology, Sexual Medicine and STDs, Faculty of Medicine, Helwan University, Cairo, Egypt.
¹² Department of Dermatology and Andrology, Armed Forces College of medicine, Cairo, Egypt.

Abstract

Background: Vitamin D is a regulatory factor for skin immune functions through vitamin D receptor, which is expressed on many immune cells. Vitamin D receptor is located on chromosome 12q 13.11 and has many single nucleotide polymorphisms. Some of them were hypothesized to be associated with psoriasis. Psoriasis is a genetic disease that is greatly affected by environmental factors.

Methods: A total of 135 psoriasis patients and 114 healthy controls were recruited. Both had a measurement of serum vitamin D and two vitamin D receptor variants:, rs1544410: G > A (HGVS:NC_000012.12:g.47846052) and rs7975232: C > A (HGVS: NC_000012.12:g.47845054). We assessed the relationship between vitamin deficiency as well as the two gene polymorphisms with psoriasis susceptibility and severity.

Results: Serum vitamin D levels were not significantly different between cases and controls. However, a significant association between vitamin D levels and severity was observed. We attributed this to our finding that rs7975232 was more significantly polymorphic among cases than controls, while rs1544410 polymorphism did not show a significant difference among the 2 groups.

Conclusion: We did not find a significant difference in serum vitamin D levels between cases and controls. Yet, psoriasis severity was significantly associated with serum vitamin D levels. We attributed this to other findings that the vitamin D receptor rs7975232 gene is polymorphic in psoriasis patients. At the same time, rs1544410 was not significantly more polymorphic in psoriasis patients. Both genes' polymorphisms were associated with severe psoriasis.

Keywords: ApaI; BsmI; psoriasis; rs1544410; rs7975232; single nucleotide polymorphism; vitamin D deficiency; vitamin D receptors polymorphisms.