A Freeze-Dried Cranberry Powder Consistently Enhances SCFA Production and Lowers Abundance of Opportunistic Pathogens In Vitro

Christina Khoo; Cindy Duysburgh; Massimo Marzorati; Pieter Van den Abbeele; Derek Zhang

doi:10.3390/biotech11020014

A Freeze-Dried Cranberry Powder Consistently Enhances SCFA Production and Lowers Abundance of Opportunistic Pathogens In Vitro

BioTech (Basel). 2022 May 6;11(2):14. doi: 10.3390/biotech11020014.

Authors

Christina Khoo¹, Cindy Duysburgh², Massimo Marzorati^{2

3}, Pieter Van den Abbeele^{2

4}, Derek Zhang^{1

5}

Affiliations

¹ Ocean Spray Cranberries, Inc., Bridge Street 152, Middleborough, MA 02349, USA.
² ProDigest BV, Technologiepark-Zwijnaarde 73, 9052 Ghent, Belgium.
³ Center of Microbial Ecology and Technology (CMET), Ghent University, 9000 Ghent, Belgium.
⁴ Cryptobiotix SA, Technologiepark-Zwijnaarde 82, 9052 Ghent, Belgium.
⁵ IQVIA, Emperor Boulevard 4820, Durham, NC 27703, USA.

Abstract

The American cranberry, Vaccinium macrocarpon, contains fibers and (poly)phenols that could exert health-promoting effects through modulation of gut microbiota. This study aimed to investigate how a freeze-dried whole cranberry powder (FCP) modulated metabolite production and microbial composition using both a 48-h incubation strategy and a long-term human gut simulator study with the M-SHIME (Mucosal Simulator of the Human Intestinal Microbial Ecosystem). FCP was repeatedly administered over three weeks. The studies included five and three study subjects, respectively. In both models, FCP significantly increased levels of health-related short-chain fatty acids (SCFA: acetate, propionate and butyrate), while decreased levels of branched-chain fatty acids (markers of proteolytic fermentation). Interestingly, FCP consistently increased luminal Bacteroidetes abundances in the proximal colon of the M-SHIME (+17.5 ± 9.3%) at the expense of Proteobacteria (-10.2 ± 1.5%). At family level, this was due to the stimulation of Bacteroidaceae and Prevotellaceae and a decrease of Pseudomonodaceae and Enterobacteriaceae. Despite of interpersonal differences, FCP also increased the abundance of families of known butyrate producers. Overall, FCP displayed an interesting prebiotic potential in vitro given its selective utilization by host microorganisms and potential health-related effects on inhibition of pathogens and selective stimulation of beneficial metabolites.

Keywords: Akkermansia muciniphila; SCFA; cranberry; in vitro; pathogen; prebiotic.

Grants and funding

The studies described in this manuscript were performed by ProDigest at the request of and were funded by Ocean Spray Cranberries, Inc. Ocean Spray Cranberries, Inc also funded Cryptobiotix to facilitate the conceptualization and writing of the manuscript.