A Detached Leaf Assay for Rapidly Screening Plant Pathogen-Biological Control Agents

Giovana Prado Fortuna Macan; Sammar Khalil; Pruthvi B Kalyandurg; Nidhi Pareek; Ramesh R Vetukuri

doi:10.1007/978-1-0716-2517-0_26

A Detached Leaf Assay for Rapidly Screening Plant Pathogen-Biological Control Agents

Methods Mol Biol. 2022:2536:449-458. doi: 10.1007/978-1-0716-2517-0_26.

Authors

Giovana Prado Fortuna Macan¹, Sammar Khalil², Pruthvi B Kalyandurg¹, Nidhi Pareek³, Ramesh R Vetukuri⁴

Affiliations

¹ Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden.
² Department of Biosystems and Technology, Swedish University of Agricultural Sciences, Alnarp, Sweden.
³ Department of Microbiology, School of Life Sciences, Central University of Rajasthan, Bandarsindri, Kishangarh, Ajmer, Rajasthan, India.
⁴ Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden. Ramesh.Vetukuri@slu.se.

PMID: 35819620
DOI: 10.1007/978-1-0716-2517-0_26

Abstract

The detached leaf assay (DLA) is a nondestructive method for evaluating interactions between plants and disease-causing agents that allows quick characterization of potential pathogens' infectivity and plants' resistance to them. Here we show its utility for also assessing potential biological control agents (BCAs), by demonstrating its applicability for screening potential BCAs for the strawberry (Fragaria × ananassa) pathogen Phytophtora cactorum.

Keywords: Antagonist; Biological control agents (BCA); Detached leaf assay; Phytophtora cactorum; Plant disease; Screening.

MeSH terms

Biological Control Agents*
Fragaria*
Plant Diseases
Plant Leaves
Research

Substances

Biological Control Agents