Innovative Detection of the Quarantine Plant Pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens, Causal Agent of Bacterial Wilt of Leguminous Plants

Stefania Tegli; Dario Gaudioso; Damiano Stefanucci

doi:10.1007/978-1-0716-2517-0_15

Innovative Detection of the Quarantine Plant Pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens, Causal Agent of Bacterial Wilt of Leguminous Plants

Methods Mol Biol. 2022:2536:251-261. doi: 10.1007/978-1-0716-2517-0_15.

Authors

Stefania Tegli¹, Dario Gaudioso², Damiano Stefanucci²

Affiliations

¹ Department of Agriculture, Food, Environment and Forestry (DAGRI), Molecular Plant Pathology Lab, University of Florence, Florence, Italy. stefania.tegli@unifi.it.
² Department of Agriculture, Food, Environment and Forestry (DAGRI), Molecular Plant Pathology Lab, University of Florence, Florence, Italy.

PMID: 35819609
DOI: 10.1007/978-1-0716-2517-0_15

Abstract

Specific, sensitive, and rapid detection of quarantine and regulated plant pathogens is pivotal for the control of the diseases they cause. Here, we describe the PCR-based methods which have been developed for Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff), quarantine plant pathogenic bacterium for EU and causal agent of bacterial wilt of Leguminous plants. These methods include an end-point and a real-time PCR test, and a LAMP assay. Their threshold analytical limits range from 100 to 10 fg of DNA template per reaction and are currently used worldwide for routine testing for Cff from laboratory to field scale.

Keywords: Bacterial wilt of bean; Curtobacterium flaccumfaciens pv. flaccumfaciens; End-point PCR; Visual LAMP; qPCR; rep-PCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actinobacteria
Actinomycetales*
Fabaceae* / microbiology
Plant Diseases / microbiology
Quarantine
Vegetables

Supplementary concepts

Curtobacterium flaccumfaciens