DNA extraction from plant samples is very important for a good performance of diagnostic molecular assays in phytopathology. The variety of matrices (such as leaves, roots, and twigs) requires a differentiated approach to DNA extraction. Here we describe three categories of matrices: (a) symptomatic bark/wood tissue; (b) residues of frass resulting from insect woody trophic activities, portions of the galleries produced in the wood, and tissues surrounding exit holes; and (c) leaves of different plant species. To improve the performances of diagnostic assays, we here describe DNA extraction procedures that have been optimized for each matrix type.
Keywords: Diagnostic tools; Invasive pathogen; Molecular identification; Plant pathogens; Quarantine pests; Xylophagous insects.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.