Trophic niche partitioning of five sympatric shark species in the tropical eastern Pacific Ocean revealed by multi-tissue fatty acid analysis

Abstract

Fatty acid (FA) analysis of consumer tissues has recently shown utility in drawing further inferences about trophic niche dynamics of marine predators such as sharks. In this study, we examined liver, plasma, and muscle FAs in five coexisting pelagic sharks (blue (Prionace glauca), silky (Carcharhinus falciformis), bigeye thresher (Alopias superciliosus), pelagic thresher (Alopias pelagicus), and smooth hammerhead (Sphyrna zygaena)) inhabiting the tropical eastern Pacific Ocean. Results showed complex inter- and intra-individual and tissue variation among the five shark species. Based on multivariate analysis of the muscle FAs, P. glauca and C. falciformis have the largest FA niche widths, indicating diverse feeding habits or habitat isolation, whereas A. pelagicus and S. zygaena occupied a narrower niche width, reflecting increased trophic specialization. High percentages of muscle FA niche overlap indicated strong resource competition between S.zygaena and C. falciformis and a degree of dietary isolation by P. glauca. Interpretations of feeding ecology differed based on the analysis of plasma FAs, which could be attributed to higher dietary FA turnover rates. The liver was deemed unsuitable to examine FA niche metrics based on high and unexplained intra-specific variance in liver FAs as well as the unique lipid metabolism in chondrichthyans. Overall, our multi-tissue approach revealed the magnitude of potential competitive interactions among coexisting tropical shark species. It also expanded our understanding of inter-tissue variability and best practices when using FA analysis to estimate trophic niche metrics of sharks.

Keywords: Fatty acids; Feeding ecology; Multiple tissues; Pelagic shark; Species coexistence.