Belowground Carbon Efficiency for Nitrogen and Phosphorus Acquisition Varies Between Lolium perenne and Trifolium repens and Depends on Phosphorus Fertilization

Jiayu Lu; Jinfeng Yang; Claudia Keitel; Liming Yin; Peng Wang; Weixin Cheng; Feike A Dijkstra

doi:10.3389/fpls.2022.927435

Belowground Carbon Efficiency for Nitrogen and Phosphorus Acquisition Varies Between Lolium perenne and Trifolium repens and Depends on Phosphorus Fertilization

Front Plant Sci. 2022 Jun 24:13:927435. doi: 10.3389/fpls.2022.927435. eCollection 2022.

Authors

Jiayu Lu^{1

2}, Jinfeng Yang³, Claudia Keitel², Liming Yin¹, Peng Wang¹, Weixin Cheng⁴, Feike A Dijkstra²

Affiliations

¹ CAS Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China.
² School of Life and Environmental Sciences, Sydney Institute of Agriculture, The University of Sydney, Sydney, NSW, Australia.
³ National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Land and Environment, Shenyang Agricultural University, Shenyang, China.
⁴ Environmental Studies Department, University of California, Santa Cruz, Santa Cruz, CA, United States.

Abstract

Photosynthetically derived carbon (C) is allocated belowground, allowing plants to obtain nutrients. However, less is known about the amount of nutrients acquired relative to the C allocated belowground, which is referred to as C efficiency for nutrient acquisition (CENA). Here, we examined how C efficiency for nitrogen (N) and phosphorus (P) acquisition varied between ryegrass (Lolium perenne) and clover (Trifolium repens) with and without P fertilization. A continuous ¹³C-labeling method was applied to track belowground C allocation. Both species allocated nearly half of belowground C to rhizosphere respiration (49%), followed by root biomass (37%), and rhizodeposition (14%). With regard to N and P, CENA was higher for clover than for ryegrass, which remained higher after accounting for relatively low C costs associated with biological N₂ fixation. Phosphorus fertilization increased the C efficiency for P acquisition but decreased the C efficiency for N acquisition. A higher CENA for N and P in clover may be attributed to the greater rhizosphere priming on soil organic matter decomposition. Increased P availability with P fertilization could induce lower C allocation for P uptake but exacerbate soil N limitation, thereby making N uptake less C efficient. Overall, our study revealed that species-specific belowground C allocation and nutrient uptake efficiency depend on which nutrient is limited.

Keywords: 13C-labeling; belowground carbon allocation; biological nitrogen fixation; carbon allocation for nutrient uptake; rhizosphere priming effect.