Genome-wide transcriptome profiling of CSF virus challenged monocyte-derived macrophages provides distinct insights into immune response of Landrace and indigenous Ghurrah pigs

Harish Kumar K P; Amit Kumar; Shikha Saxena; Arnav Mehrotra; Sheikh Firdous Ahmad; Basavaraj Sajjanar; Sudhir Srivastava; Waseem Akram Malla; Anuj Chauhan; Pronab Dhar; Bishnu Prasad Mishra; Triveni Dutt; Raj Kumar Singh

doi:10.1016/j.ygeno.2022.110427

Genome-wide transcriptome profiling of CSF virus challenged monocyte-derived macrophages provides distinct insights into immune response of Landrace and indigenous Ghurrah pigs

Genomics. 2022 Jul;114(4):110427. doi: 10.1016/j.ygeno.2022.110427. Epub 2022 Jul 6.

Affiliations

¹ Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, U.P, India.
² Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, U.P, India. Electronic address: vetamitchandan07@gmail.com.
³ Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, U.P, India.
⁴ ICAR-Indian Agricultural Statistics Research Institute, New Delhi, Delhi 110012, India.
⁵ Livestock Production and Management Section, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, U.P, India.
⁶ Biological Standardization Division, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, U.P, India.

PMID: 35803450
DOI: 10.1016/j.ygeno.2022.110427

Abstract

The present study was undertaken to characterize the distinct immune response in indigenous Ghurrah and exotic Landrace pigs by challenging monocyte-derived macrophages (MDMs) with CSF virus under in-vitro conditions and assessing the variations in the transcriptome profile at 48 h post-infection (hpi). RNA-sequencing was carried out in infected and non-infected MDMs of Ghurrah (n = 3) and Landrace (n = 3) piglets prior- as well as post-stimulation. MDMs of Ghurrah showed greater immune regulation in response to CSF infection with 518 significantly differentially expressed genes (DEG) in infected versus non-infected MDMs, as compared to only 31 DEGs in Landrace MDMs. In Landrace, the principal regulators of inflammation (IL1α, IL1β and TNF) were upregulated in infected cells while in Ghurrah, these were downregulated. Overall, macrophages from indigenous Ghurrah showed more immunological dysregulation in response to virulent CSF virus infection as compared to the exotic Landrace pigs.

Keywords: CSF; Differential Immune response; In-vitro challenge; Monocyte-derived macrophages; Transcriptome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Gene Expression Profiling*
Immunity
Macrophages*
Swine
Transcriptome