Potassium ion regulates hormone, Ca2+ and H2O2 signal transduction and antioxidant activities to improve salt stress resistance in tobacco

Abstract

Although improvement of plant salt tolerance by potassium ions (K⁺) has been widely studied, whether the tolerance is mediated via hormone signaling or antioxidant systems remains to be explored. This study combined plant physiology with transcriptomic techniques to study how K⁺ interacts with hormones and antioxidant enzymes to improve plant salt tolerance. Tobacco was used as the test material to study the effects of exogenous potassium application on photosynthetic function, hormone signal transduction, and reactive oxygen species (ROS) production under NaCl stress. The study also evaluated the function of the Ca²⁺ signaling pathway in salt stress tolerance. Transcriptome data showed that 4413 up-regulated genes and 3743 down-regulated genes were found in tobacco leaves treated with NaCl compared with the control. Compared with NaCl, the down-regulated genes in tobacco leaves were significantly reduced under NaCl + KCL treatment. The results showed that NaCl stress caused oxidative damage to tobacco leaves due to increased superoxide anion (O₂^-) content, superoxide dismutase (SOD) dismutates superoxide anion to produce hydrogen peroxide and the accumulation of H₂O₂ caused by reduced ascorbate peroxidase (APX) and peroxidase (POD) activities. NaCl stress also increased abscisic acid (ABA) content in tobacco leaves, resulting in stomatal closure and reduced photosynthetic capacity. Transcriptome data showed that 5 SOD, 1 POD, 1 CAT, 5 APX, and 3 GPX genes were significantly down-regulated by the NaCl treatment. Contrarily, NaCl + KCl treatment reduced the accumulation of O₂^-and SOD activity but increased POD activity, thereby reducing the accumulation of H₂O₂ and alleviating oxidative damage. The expression of 2 SOD and 3 APX and 2 GPX genes was significantly higher in NaCl + KCl treatment than that in NaCl treatment. Sufficient K⁺ also increased indole acetic acid (IAA) levels in tobacco leaves under NaCl stress but reduced ABA content, promoting stomatal opening and improving the photosynthetic capacity. In conclusion, K⁺ can improve plant salt tolerance by alleviating oxidative damage and regulating hormone signal transduction.

Keywords: Antioxidant enzymes; Calcium signaling; Hormone signaling; Potassium ions; Salt stress.