The Molecular Mechanism of Yellow Mushroom (Floccularia luteovirens) Response to Strong Ultraviolet Radiation on the Qinghai-Tibet Plateau

Jing Guo; Zhanling Xie; Hongchen Jiang; Hongyan Xu; Baolong Liu; Qing Meng; Qingqing Peng; Yongpeng Tang; Yingzhu Duan

doi:10.3389/fmicb.2022.918491

The Molecular Mechanism of Yellow Mushroom (Floccularia luteovirens) Response to Strong Ultraviolet Radiation on the Qinghai-Tibet Plateau

Front Microbiol. 2022 Jun 20:13:918491. doi: 10.3389/fmicb.2022.918491. eCollection 2022.

Authors

Jing Guo^{1

2}, Zhanling Xie^{1

2}, Hongchen Jiang^{3

4}, Hongyan Xu^{1

5}, Baolong Liu⁶, Qing Meng^{1

2}, Qingqing Peng^{1

2}, Yongpeng Tang⁷, Yingzhu Duan⁸

Affiliations

¹ College of Ecological and Environment Engineering, Qinghai University, Xining, China.
² State Key Laboratory Breeding Base for Innovation and Utilization of Plateau Crop Germplasm, Qinghai University, Xining, China.
³ State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, China.
⁴ Qinghai Provincial Key Laboratory of Geology and Environment of Salt Lakes, Qinghai Institute of Salt Lakes, Chinese Academy of Sciences, Xining, China.
⁵ Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, China.
⁶ Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.
⁷ Bureau of Forestry and Grassland, Delingha, China.
⁸ Test Station for Grassland Improvement, Xining, China.

Abstract

The Qinghai-Tibet Plateau (QTP) is the highest plateau in the world, and its ultraviolet (UV) radiation is much greater than that of other regions in the world. Yellow mushroom (Floccularia luteovirens) is a unique and widely distributed edible fungus on the QTP. However, the molecular mechanism of F. luteovirens's response to strong UV radiation remains unclear. Herein, we reported the 205 environmental adaptation and information processing genes from genome of F. luteovirens. In addition, we assembled the RNA sequence of UV-affected F. luteovirens at different growth stages. The results showed that in response to strong UV radiation, a total of 11,871 significantly different genes were identified, of which 4,444 genes in the vegetative mycelium (VM) stage were significantly different from the young fruiting bodies (YFB) stage, and only 2,431 genes in the YFB stage were significantly different from fruiting bodies (FB) stage. A total of 225 differentially expressed genes (DEGs) were found to be involved in environmental signal transduction, biochemical reaction preparation and stress response pathway, pigment metabolism pathway, and growth cycle regulation, so as to sense UV radiation, promote repair damage, regulate intracellular homeostasis, and reduce oxidative damage of UV radiation. On the basis of these results, a molecular regulation model was proposed for the response of F. luteovirens to strong UV radiation. These results revealed the molecular mechanism of adaptation of F. luteovirens adapting to strong UV radiation, and provided novel insights into mechanisms of fungi adapting to extreme environmental conditions on the QTP; the production the riboflavin pigment of the endemic fungi (Yellow mushroom) in the QTP was one of the response to extreme environment of the strong UV radiation.

Keywords: Floccularia luteovirens; Qinghai-Tibet Plateau; chlorophyll; molecular regulation model; riboflavin; strong UV radiation.